化工新型材料

化工新型材料 ›› 2020, Vol. 48 ›› Issue (4) : 241-244.

开发与应用

端羧基季铵化壳聚糖的制备及抗菌性能研究

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作者信息 +

Preparation and antibacterial performance of CTMC

Wang Fei^1,2, Zhang Yisheng^1,2, Xue Wenman^1,2, Guo Ruijie^1,3, Niu Baolong^1,3, Wang Huifang^1,2

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文章历史 +

Received
2018-11-30
Issue Date	Revised Date
2020-05-14	2018-11-30

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摘要

以脱乙酰度90%的壳聚糖为原料,采用两步合成方法,得到N,N,N-三甲基壳聚糖(TMC),将TMC与氯乙酸反应,得到端羧基季铵化壳聚糖(CTMC),利用傅里叶变换红外光谱仪和核磁共振氢谱仪对产物进行了表征并研究其对大肠杆菌和金黄色葡萄球菌的抗菌性能。结果表明:通过两步法成功合成了CTMC,实现了对壳聚糖分子结构的改性;CTMC的溶解性得到明显提高,其可以溶解于任意pH的水溶液中,且在中性溶液中表现出一定的抗菌性。CTMC的抗菌机理可能主要是带正电的壳聚糖分子链与带负电的细菌细胞壁存在静电作用,改变了细胞壁的通透性,影响细胞内外物质传递从而杀死细菌。

Abstract

N,N,N-trimethyl chitosan (TMC) was successfully synthesized making use of two steps method,and carboxymethyl-quaternized chitosan(CTMC) was further obtained by TMC reacted with monobromoacetic acid.The products were characterized and their performance against E.coli and S.aureus were studied.The results indicated that CTMC was successfully synthesized and the molecular structure of CS was modified,CTMC could dissolve in aqueous solution with any pH value and exhibited antibacterial activity in neutral solution.In addition,the antibacterial mechanism may be that the positive charged chitosan can adsorb to the negative charged surface of bacterial,which changed the permeability of the cell wall,influenced the cytoplasm flow of the cell,and finally kill bacteria.

关键词

壳聚糖 / 结构表征 / 溶解性 / 抗菌性 / 抗菌机制

Key words

chitosan / structure characterization / solubility / antibacterial property / antibacterial mechanism

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端羧基季铵化壳聚糖的制备及抗菌性能研究[J]. 化工新型材料, 2020, 48(4): 241-244 DOI:

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参考文献

原文顺序 | 出版日期 | 本文引用

[1] Kumirska J,Weinhold M X,Jorg Thoming J,et al.Biomedical activity of chitin/chitosan based materials—influence of physicochemical properties apart from molecular weight and degree of N-acetylation[J].Polymers,2011,3(4):1875-1901.
[2] 宋丽丽,高亚男,陈思敏,等.壳聚糖的化学改性及其衍生物的抗氧化活性研究进展[J].中国药师,2018,2(21):320-323.
[3] 崔文慧,郭芹,李庆鹏,等.壳聚糖及壳聚糖-柠檬酸结构性质和功能性质的比较[J].食品科学,2017,38(5):134-141.
[4] Karimi A,Shahrooz R,Hobbenaghi R,et al.Mouse bone marrow-derived mast cells induce angiogenesis by tissue engineering in rats:histological evidence[J].Cell Journal,2018,19(4):578-584.
[5] Sharmeen S,Rahman A F M M,Lubna M M,et al.Polyethylene glycol functionalized carbon nanotubes/gelatin-chitosan nanocomposite:an approach for significant drug release[J].Bioactive Materials,2018,3(3):236-244.
[6] 钱宇,汪慧超,薛秀恒,等.壳聚糖甘油二酯微乳液的制备、表征及稳定性研究[J].中国油脂,2017,42(11):28-32.
[7] Anisha B S,Sankar D,Mohandas A,et al.Chitosan-hyaluronan/nano chondroitin sulfate ternary composite sponges for medical use[J].Carbohydr Polym,2013,92(2):1470-1476.
[8] 龙德武,吴国忠,秦宗英,等.辐射法降解壳聚糖及其抑菌性能的研究[J].高分子材料科学与工程,2005,21(6):240-242.
[9] Avadi M R,Mahdavinia G,Sadeghi A M,et al.Synthesis and characterization of N-diethyl methyl chitosan[J].Iranian Polymer Journal(English Edition),2004,13(5):431-436.
[10] Patrulea V,Applegate L A,Ostafe V,et al.Optimized synthesis of O-carboxymethyl-N,N,N-trimethyl chitosan[J].Carbohydrate Polymers,2015,122:46-52.
[11] He G,Chen X,Yin Y,et al.Synthesis,characterization and antibacterial activity of salicyloyl chitosan[J].Carbohydrate Polymers,2011,83(3):1274-1278.
[12] Li B,Shan C L,Zhou Q,et al.Synthesis,characterization,and antibacterial activity of cross-linked chitosan-glutaraldehyde[J].Marine Drugs,2013,11(5):1534-1552.
[13] Tang H,Zhang P,Kieft T L,et al.Antibacterial action of a novel functionalized chitosan-arginine against gram-negative bacteria[J].Acta Biomaterialia,2010,6(7):2562-2571.
[14] Khalil E S,Saad B,Negim E S M,et al.Novel water-soluble chitosan derivative prepared by graft polymerization of dicyandiamide:synthesis,characterisation,and its antibacterial property[J].Journal of Polymer Research,2015,22(6):116.
[15] 董卫民,左华江,吴丁财,等.季铵盐高分子抗菌剂的工艺优化与抗菌性能研究[J].离子交换与吸附,2011,27(1):1.
[16] Xiao B,Wan Y,Zhao M,et al.Preparation and characterization of antimicrobial chitosan-N-arginine with different degrees of substitution[J].Carbohydrate Polymers,2011,83(1):144-150.