聚丙烯酰胺 (PAM)经三步反应被很好地接枝到载玻片上,又特别针对反应体系中水对产物形貌的影响做了研究。通过使用X射线光电子能谱 (XPS)、原子力显微镜 (AFM)和扫描电镜 (SEM)等手段对成功接枝PAM后的玻片进行测试。结果表明:反应体系中水的存在与否,会导致两种差异很大的结构和组成成分,水存在时,载玻片上生成了形貌结构为两层的胺基聚合物,且上层胺基密度不一。水不存在时,其表面生成了单层分子结构的胺基聚合物,同时其密度更加均一。
Poly acrylamide (PAM)was successfully grafted onto the glass slide surface by three steps reaction.Especially the effect of water on the surface structure of the product was investigated.X-ray photo electron spectroscopy (XPS),atomic force microscope (AFM) and scanning electron microscopy (SEM) were utilized to characterize the surface composition and morphology of the surface.As a result,there were two completely different structures and surface compositions under anhydrous and anhydrous conditions.Consequently,the amino polymer formed on the surface of the glass slide was divided into two layers in the presence of water,and upper amine group density was different.While the amino polymer formed on the glass slide surface formed a mono-layer structure without water,as the same time the amine group density was more uniform.
[1] 相森.玻璃表面修饰的基底材料对细胞粘附研究[D].北京:北京化工大学,2012.
[2] 付先利,支月莹,郑芳,等.四种制作多聚赖氨酸免疫组化黏附载玻片方法的比较[J].临床与实验病理学杂志,2016,32 (17):347-348.
[3] Tsougeni K,Ellinas K,Koukouvinos G,et al.Three-dimensional (3D) plasma micro-nanotextured slides for high performance biomolecule microarrays:comparison with epoxy-silane coated glass slides[J].Colloids & Surfaces B Biointerfaces,2018,165:270-277.
[4] 赵平英,苗伟俊,刘峰,等.金属表面硅烷化技术概述[J].化工新型材料,2014,42 (4):36-37,46.
[5] 郑建华,张晓凯,卢慧粉.特定条件下的定向ZnO纳米棒的制备及表征[J].化学学报,2011,69 (20):2434-2438.
[6] 马菲,周林,王薇,等.二氧化硅纳米粒载体固定血红蛋白的制备及性能研究[C].厦门:中国化学会第27届学术年会第03分会场,2010.
[7] Lan S D,Cheng C C,Huang C H,et al.Synthesis of sub-10nm VO2,nanoparticles films with plasma-treated glass slides by aqueous sol-gel method[J].Applied Surface Science,2015,357 (21):2069-2076.
[8] 朱滨,景奉香,赵建龙,等.用氨基修饰的载玻片制作cDNA微阵列[J].生物化学与生物物理进展,2001,28 (1):121-124.
[9] 唐姗.化学合成光控蛋白探针用于研究免疫细胞信号传导机制[D].北京:清华大学,2016.
[10] Manzoor O,Soleja N,Mohsin M.Nanoscale gizmos-the novel fluorescent probes for monitoring protein activity[J].Biochemical Engineering Journal,2018,133 (3):83-95.
[11] 王普红,余建华,李志军,等.基于液晶取向变化检测有机胺化合物的方法[J].分析化学,2012,40 (5):796-800.
[12] 费嘉,马文丽,吴清华,等.介质表面修饰对蛋白质芯片固定率和反应性的影响[J].生命科学研究,2005,9 (4):341-345.
[13] 赵剑英,李玉邯,郭海全,等.生物芯片表面氨基密度检测及稳定性研究[J].分析化学,2006,34 (9):1235-1238.
[14] Graczyk T,Hornof V.Effect of stirring on cellulose graft copolymerizaton[J].J Appl Polym Sci,1983,28 (16):1371-1378.
基金资助
国家自然科学基金(51603020);吉林省教育厅(JJKH20170555KJ)
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