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摘要
以氨水为交联剂,冰乙酸为改性剂,生物相容性良好的可降解生物材料魔芋葡甘聚糖(KGM)、壳聚糖(CS)和纳米羟基磷灰石(纳米-HAP)为基本原料,在碱性溶液环境下将三者机械混合得到凝胶,经熟化、除碱和冷冻干燥等工艺手段,制备得到魔芋葡甘聚糖/纳米羟基磷灰石/壳聚糖(KHC)复合多孔支架。通过抗压强度和气孔率测试,以及X射线衍射、傅里叶红外光谱和扫描电镜等检测手段探讨KGM含量对复合支架材料结构与物化性能的影响,其后对实验较优组进行CS酸改性处理,并与未改性组进行对比分析。结果表明:当KGM含量为原比例(1KGM)时,支架材料有机相包裹无机相的能力最好,当KGM含量大于原比例(5/4KGM、3/2KGM)时,有机相包裹能力逐渐减弱;原比例组CS经酸改性后支架的包裹性能将进一步提升,酸改性组支架抗压强度最高,达到了1.1MPa。
Abstract
Ammonia used as crosslinking agent,glacical acetic acid as modifier,and konjac glucomannan(KGM)/chistosan(CS)/nano-hydroxyapatite (nano-HAP) with good biocompatibility as raw materials,the gel with three components was prepared by magnetic stirring in the alkaline solution.Finally,the konjac glucomannan/nano-hydroxyapatite/chitosan (KHC) composite scaffold was prepared by the steps of aging,alkaline removing and freezing-drying.The effect of KGM content on the physical and chemical property were investigated by compressive strength test,porosity test,XRD,FT-IR and SEM.The experimental group was also investigated between CS modified with acid and unmodified.The results indicated that when KGM content was in the level of 1KGM,the organic phase of scaffold had the best ability to encapsulate the inorganic phase.When KGM content was higher than the original proportion (5/4KGM,3/2KGM),the encapsulation capacity of organic phase decreased.It was also indicated that the ability of encapsulate could be further promoted by the acid modification,and the acid-modified group had the highest compressive strength,which can be reached to 1.1MPa.
关键词
生物相容性
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魔芋葡甘聚糖
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机械混合
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支架材料
Key words
biocompatibility
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konjal glucomannan
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mechanical blending
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scaffold material
有机相对魔芋葡甘聚糖/纳米羟基磷灰石/壳聚糖复合多孔骨组织工程支架材料性能的影响研究[J].
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基金资助
国家自然科学基金(31260228);云南省自然科学基金国际合作项目(2011IA008)
