栅格型金纳米颗粒-聚二甲基硅氧烷(AuNP-PDMS)复合薄膜具有较大的比表面积,可以提高传感器的灵敏度,被广泛应用于生物传感领域。通过3D打印技术,实现了栅格型AuNP-PDMS的制备,通过原位还原和葡萄糖还原的方法制备了栅格型AuNPs-PDMS复合薄膜。通过扫描电镜图,揭示了金纳米层在PDMS中的均匀分布。基于栅格型AuNPs-PDMS复合薄膜的表面应力可以实现0～50ng/mL浓度范围的癌胚抗原(CEA)检测,检测限为0.412ng/mL。同时传感器对CEA检测表现出良好的特异性、选择性和稳定性。
Grid type gold nanoparticle-polydimethylsiloxane (AuNP-PDMS) composite films have large specific surface areas,which can improve the sensitivity of sensors,and thus have been widely used in the field of biosensing.The preparation of grid type PDMS was achieved through 3D printing technology,and grid type AuNPs-PDMS composite films were prepared by in-situ reduction and glucose reduction methods.The uniform distribution of gold nanolayers in PDMS was revealed through SEM images.The surface stress of the grid type AuNPs-PDMS composite film could achieve CEA detection in the concentration range of 0～50ng/mL,with a detection limit of 0.412ng/mL.At the same time,the sensor exhibited good specificity,selectivity,and stability in detecting CEA.
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基金资助
山西省中央引导地方科技发展资金项目(YDZJSX2021A018);山西省基础研究计划青年科学研究项目(20210302124557)
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