缺氧环境是实体瘤的显著特征之一,在诸多生理病理进程中发挥着关键作用,因此对其进行精准检测意义重大。近年来,荧光探针凭借高灵敏度、实时监测及原位成像等优势,成为缺氧检测领域的研究焦点。其中,氟硼二吡咯化合物(BODIPY)作为荧光团,以其优异的光学性能、良好的光稳定性和易于修饰的结构,在缺氧检测荧光探针领域得到了广泛应用。着重阐述了各类基于BODIPY的缺氧探针的构建策略、识别机制及其在生物成像中的应用,并对当前面临的挑战,诸如生物相容性、体内复杂环境干扰等问题进行总结。目的在于启发荧光探针对缺氧环境成像的新方法与创新思路,推动肿瘤诊断及缺氧反应性抗癌药物的发展。
The hypoxic environment is one of the remarkable characteristics of solid tumors and plays a crucial role in many physiological and pathological processes.Therefore,accurate detection of hypoxia is of great significance.In recent years,fluorescence probes have become the research focus in the field of hypoxia detection due to their advantages such as high sensitivity,real-time monitoring,and in-situ imaging.Among them,boron dipyrromethene (BODIPY) as a fluorophore has been widely used in the field of fluorescence probes for hypoxia detection owing to its excellent optical properties,good photostability,and easily modifiable structure.We elaborated on the construction strategies,recognition mechanisms,and applications in bioimaging of various BODIPY-based hypoxia probes,and summarized the current challenges,such as biocompatibility and interference from the complex in vivo environment.The aim is to inspire new methods and innovative ideas for fluorescence probes to image the hypoxic environment,and promote the development of tumor diagnosis and hypoxia-responsive anticancer drugs.
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基金资助
河南省科技攻关项目(242102231037);河南省郑州轻工业大学博士启动基金(2021BSJJ010)
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