PDF
摘要
以无水氯化铜和硫代乙酰胺分别为铜源和硫源合成硫化铜纳米球(CuSNSs),并成功在其表面负载了银纳米粒子(AgNPs),得到CuSNSs/AgNPs复合材料。用扫描电子显微镜对其进行形貌表征,并用X射线衍射仪分析成分。基于CuSNSs和AgNPs的协同作用,该复合材料修饰电极对过氧化氢还原具有较好的电催化性能。将其与链霉亲和素(SA)结合,得到CuSNSs/AgNPs/SA信号探针,用于黄曲霉素B1(AFB1)电化学适配体传感器的构建。基于竞争分析原理,实现了AFB1的定量检测。该传感器检测AFB1的线性范围为0.1pmol/L～500nmol/L,检出限为0.03pmol/L,且具有较好的重现性和稳定性。将该传感器用于花生和牛奶样品中AFB1的检测,回收率范围为97.5%～104.3%,表明其准确度好,可用于实际样品中AFB1的分析。
Abstract
Copper sulfide nanospheres (CuSNSs) were synthesized using copper chloride and thioacetamide as copper and sulfur sources,respectively,and silver nanoparticles (AgNPs) were successfully loaded onto the CuSNSs surface to obtain a CuSNSs/AgNPs composite.The morphology of the CuSNSs/AgNPs composite was characterized using scanning electron microscopy,and its composition was analyzed using X-ray diffraction.Based on the synergistic effect of CuSNSs and AgNPs,the CuSNSs/AgNPs composite modified electrode had good electrocatalytic ability for hydrogen peroxide reduction.The CuSNSs/AgNPs composite was combined with streptavidin (SA) to obtain a CuSNSs/AgNPs/SA signal probe for the construction of an electrochemical aptasensor for aflatoxin B1 (AFB1).Based on the principle of competitive analysis,quantitative detection of AFB1 was achieved.The linear range of this sensor for detecting AFB1 was 0.1pmol/L to 500nmol/L,with a detection limit of 0.03pmol/L,and it had good reproducibility and stability.The sensor was used to detect AFB1 in peanut and milk samples,with a recovery range of 97.5% to 104.3%,indicating that this method had good accuracy and the sensor could be used for AFB1 analysis in real samples.
关键词
硫化铜纳米球
/
银纳米粒子
/
复合材料
/
电化学适配体传感器
/
黄曲霉素B1
Key words
copper sulfide nanospheres
/
silver nanoparticles
/
composite
/
electrochemical aptasensor
/
aflatoxin B1
基于硫化铜纳米球/银纳米粒子复合材料的黄曲霉素B1适配体传感器研究[J].
化工新型材料, 2026, 54(1): 271-276 DOI:10.19817/j.cnki.issn1006-3536.2026.01.020
[1] 周源,吕丽云,王虹.硫化铜纳米材料的制备与应用[J].化工新型材料,2017,45(4):32-34.
[2] 刘佳佳,张凇铭,何秀芹,等.石蜡/SiO2/CuS纳米复合相变材料的制备用于太阳能的光热转换与存储[J].化工新型材料,2015,43(12):104-106.
[3] 谭鑫,谭娟,刘颜,等.基于CuS负载Pt纳米花的类过氧化物酶活性抑制检测银离子[J].分析试验室,2021,40(11):1241-1246.
[4] 蔡苗,陈子航,曾实,等.CuS纳米片修饰Bi5O7I复合材料用于光催化还原Cr(Ⅵ)水溶液[J].无机材料学报,2021,36(6):665-672.
[5] 田曼丽,田亮亮.片状Co3O4多孔材料的制备及其在葡萄糖电化学传感器中的应用[J].化工新型材料,2020,48(9):264-268.
[6] 周瑶,万起展,王燕华,等.基于Nafion共聚还原氧化石墨烯／聚苯胺复合材料的H2O2电化学传感器[J].化工新型材料,2020,48(10):192-196.
[7] 李爱学,王鹏飞.基于电化学传感器检测农副产品黄曲霉毒素B1的研究进展[J].农业工程学报,2023,39(22):258-269.
[8] Wang C,Li Y P,Zhao Q.A competitive electrochemical aptamer-based method for aflatoxin B1 detection with signal-off response[J].Anal Methods,2020,12(5):646-650.
[9] Li Y Y,Liu D,Zhu C X,et al.A ratiometry-induced successive reusable electrochemical aptasensing platform:efficient monitoring of aflatoxin B1 in peanut[J].Sens Actuators B,2021,336:129021.
[10] Wang M,Duan M T,Yu F X,et al.Development of Aflatoxin B1 aptamer sensor based on iron porphyrin organic porous material[J].Food Anal Methods,2021,14(3):537-544.
[11] Wang C Q,Qian J,An K Q,et al.A semiconductor quantum dot-based ratiometric electrochemical aptasensor for the selective and reliable determination of aflatoxin B1[J].Analyst,2019,144(16):4772-4780.
[12] 刘俊桃,刘志景,李晓江,等.基于铂@金纳米线作为信号放大物的电化学适体传感器检测黄曲霉毒素[J].食品工业科技,2023,44(24):294-300.
基金资助
常州市自然科学项目(CE20212039,CE20212030)
