以槐米提取物为原料,采用绿色合成法制备氧化锌纳米粒子(ZnO NP),通过UV-Vis、FT-IR、DLS、XRD、SEM和EDS表征产物的形貌及元素组成。结果表明,绿色合成法制得的ZnO NP呈不规则颗粒状,粒径约为235nm;采用打孔扩散法和最小抑菌浓度法(MIC)评价绿色合成ZnO NP及化学合成ZnO对大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)的抑菌活性。抑菌结果表明,ZnO NP对E.coli和S.aureus均有明显的抗菌效果,对E.coli的最小抑菌浓度(MIC)为0.250g/L,对S.aureus的MIC为0.125g/L,而化学合成ZnO对E.coli和S.aureus的MIC均为0.250g/L。
Zinc oxide nanoparticles (ZnO NP) were prepared by green synthesis method with flos sophorae immaturus extracts as raw material.The morphology and elements of the samples were analyzed by UV-Vis,FT-IR,DLS,XRD,SEM,EDS.The results showed that the samples presented irregular granular structure and the average diameter was 235nm.Well diffusion and minimum inhibitory concentration (MIC) methods were used to evaluate the antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) of ZnO NP and ZnO synthesized by green and chemical synthesis methods,respectively.The antibacterial test results showed that ZnO NP had obvious antibacterial effect against E.coli and S.aureus,and the MIC was 0.250g/L against E.coli and 0.125g/L S.aureus,while MIC of ZnO chemically synthesized both were 0.250g/L.
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基金资助
重庆市教育委员会科学技术研究项目(KJQN201801115);重庆理工大学科研启动基金资助项目(2019ZD14)
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