采用种子生长法制备金纳米颗粒,通过紫外-可见吸收光谱和透射电子显微镜对其进行表征。以金纳米颗粒作为光学探针,基于“三明治”结构核酸杂交和磁分离策略构建了对新型冠状病毒相关核酸序列的检测方法。结果表明:该方法可实现对20nmol/L目标核酸的可视化检测;通过测量溶液的紫外-可见吸收光谱,可实现对目标核酸的定量检测,检测限为0.7nmol/L。该传感方法简单且选择性好,为新型冠状病毒检测提供了新的选择。
Gold nanoparticles (AuNPs) were synthesized by gold seed growth method using hydroxylamine hydrochloride as reductant.The obtained AuNPs were characterized by UV-Vis absorption spectrum and transmission electron microscopy.Using AuNPs as optical probes,the detection method for SARS-CoV-2 related nucleic acid was constructed,which combined the sandwich-type DNA hybridization strategy and magnetic separation.Under optimized conditions,the proposed method realized 20nmol/L detection of target nucleic acid by naked eye.By measuring UV-Vis absorption spectrum of the solution,the quantitative detection was realized with detection limit of 0.7nmol/L.This sensing platform was simple and selective,which provided new choice for SARS-CoV-2 detection.
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基金资助
河南省自然科学基金青年科学基金(202300410145和202300410128);河南科技大学博士启动基金(13480042);河南省重点研发与推广专项基金(212102311038和202102310488)
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