Graphene oxide/rosmarinic acid (GO-RA) nanocomposites were prepared by grafting modified graphene oxide (GO).GO-RA nanocomposites were characterized by FT-IR,UV-Vis,XRD,TGA,SEM and TEM,Escherichia coli(E.coli) and Staphylococcus aureus(S.aureus) were used as model bacteria to comprehensively evaluate the antibacterial properties of the nanocomposite through bacterial growth inhibition experiment and bactericidal rate test.The results showed that rosmarinic acid (RA) was successfully grafted onto GO.The inhibitory effect of GO-RA on the growth and reproduction of E.coli was stronger than that of GO,and the inhibitory effect of GO-RA on S.aureus was not different from that of GO.The bactericidal rates of GO-RA exposed to E.coli and S.aureus for 2 hours were 97.6% and 94.3%,respectively.Under the same conditions,compared with GO,GO-RA showed better antibacterial activity against the above two bacteria,and the sterilization rate increased by 19.3% and 5.5%,respectively.
[1] Henriques P C,Borges I,Pinto A M,et al.Fabrication and antimicrobial performance of surfaces integrating graphene-based materials[J].Carbon,2018,132:709-732.
[2] Sharma A,Varshney M,Nanda S S,et al.Structural,electronic structure and antibacterial properties of graphene-oxide nano-sheets[J].Chemical Physics Letters,2018,698:85-92.
[3] 王林变,赵英虎,高莉,等.氧化石墨烯-纳米银复合材料的应用研究进展[J].化工新型材料,2019,47(3):1-5,10.
[4] Yousefi M,Dadashpour M,Hejazi M,et al.Anti-bacterial activity of graphene oxide as a new weapon nanomaterial to combat multidrug-resistance bacteria[J].Materials Science and Engineering:C,2017,74:568-581.
[5] 黄国家,陈志刚,李茂东,等.石墨烯和氧化石墨烯的表面功能化改性[J].化学学报,2016,74(10):789-799.
[6] Ren T,Wang Y,Yu Q,et al.Synthesis of antimicrobial peptide-grafted graphene oxide nanosheets with high antimicrobial efficacy[J].Materials Letters,2019,235:42-45.
[7] Li P,Gao Y,Sun Z,et al.Synthesis,characterization,and bactericidal evaluation of chitosan/guanidine functionalized graphene oxide composites[J].Molecules,2016,22:12.
[8] 赵欣,朱健健,李梦,等.我国抗菌剂的应用与发展现状[J].材料导报,2016,30(7):68-73.
[9] Kim GD,Park Y S,Jin YH,et al.Production and applications of rosmarinic acid and structurally related compounds[J].Applied Microbiology and Biotechnology,2015,99(5):2083-2092.
[10] 苏平,王根女,吴丹,等.迷迭香酸的生理活性功能及其来源研究进展[J].食品与发酵工业,2008,34(12):135-138.
[11] De Oliveira J R,Camargo S E A,de Oliveira L D.Rosmarinus officinalis L.(rosemary) as therapeutic and prophylactic agent[J].Journal of Biomedical Science,2019,26(1):5.
[12] Elufioye T O,Habtemariam S.Hepatoprotective effects of rosmarinic acid:insight into its mechanisms of action[J].Biomedicine & Pharmacotherapy,2019,112:108600.
[13] 秦静,姜力文,杨春苗,等.氧化石墨烯纳米银复合材料的制备及其抗菌性[J].环境化学,2016,35(3):445-450.
[14] 张璇,闫欣,黄天地,等.GO-nAg复合材料的制备及其抗菌性能研究[J].材料导报,2016,30(12):18-21,44.
[15] Singh M,Bajaj N K,Bhardwaj A,et al.Study of photocatalytic and antibacterial activities of graphene oxide nanosheets[J].Advanced Composites and Hybrid Materials,2018,1(4):759-765.
[16] 张有为,马慧玲,刘平桂,等.氧化石墨烯辐射接枝4-乙烯基吡啶及其对Cr(Ⅵ)吸附性能研究[J].化工新型材料,2018,46(12):125-128,132.
[17] 黄一石,吴朝华,杨小林.仪器分析2版[M].北京:化学工业出版社,2008:118-119.
[18] Markovic Z M,Jovanovic S P,Maskovic P Z,et al.Photo-induced antibacterial activity of four graphene based nanomaterials on a wide range of bacteria[J].RSC Advances,2018,8(55):31337-31347.
[19] 黄一石,吴朝华,杨小林.仪器分析2版[M].北京:化学工业出版社,2008:84-91.
[20] Li X,Sun J,Che Y,et al.Antibacterial properties of chitosan chloride-graphene oxide composites modified quartz sand filter media in water treatment[J].International Journal of Biological Macromolecules,2019,121:760-773.
[21] Liu T,Liu Y,Liu M,et al.Synthesis of graphene oxide-quaternary ammonium nanocomposite with synergistic antibacterial activity to promote infected wound healing[J].Burns & Trauma,2018,6(1):16.
[22] Shamsi S,Elias N,Narti Edayu Sarchio S,et al.Gallic acid loaded graphene oxide based nanoformulation (GAGO) as potential anti-bacterial agent against staphylococcus aureus[J].Materials Today:Proceedings,2018,5:S160-S165.
[23] Liu S,Zeng T H,Hofmann M,et al.Antibacterial activity of graphite,graphite oxide,graphene oxide,and reduced graphene oxide:membrane and oxidative stress[J].ACS Nano,2011,5(9):6971-6980.
[24] Gurunathan S,Han J W,Dayem A A,et al.Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa[J].Int J Nanomedicine,2012,7:5901-5914.
[25] Wu PC,Chen HH,Chen SY,et al.Graphene oxide conjugated with polymers:a study of culture condition to determine whether a bacterial growth stimulant or an antimicrobial agent?[J].Journal of Nanobiotechnology,2018,16(1):1.
[26] Kurantowicz N,Sawosz E,Jaworski S,et al.Interaction of graphene family materials with Listeria monocytogenes and Salmonella enterica[J].Nanoscale Research Letters,2015,10(1):23.
[27] 程松高.关于细菌的生长繁殖和营养要求[J].生物学通报,1960(4):173-176.
[28] 陈旭.氧化石墨烯及其二氧化硅负载药物增强抗耐药细菌活性及其机理研究[D].广州:暨南大学,2016.