酶联免疫吸附测定(ELISA)已被广泛用于临床检测、生物、食品检测和环境监测等许多领域,以玻璃为材料微流控ELISA芯片和二氧化硅包覆的磁珠均需要进行表面处理,以提高对待测分子的吸附能力。利用3-氨丙基三甲氧基硅烷和戊二醛作为交联剂对玻璃表面进行了改性处理。ELISA产物的颜色和紫外-可见分光光度计的结果均表明,改性后的玻璃对生物分子的吸附能力得到很大提升,并且解决了改性-恢复-再改性的问题。最后通过X射线光电子能谱分析了玻璃改性前后的表面化学元素的变化。
Enzyme-linked immunosorbent assay (ELISA) has been used widely in clinical diagnosis,biotechnology,food inspection and environmental monitoring.Surface modification of glass is crucial to improve the adsorption ability of the target analytes in ELISA micro-fluidic chip based on glass and magnetic beads covered silicon dioxide.The glass surface was modified by using 3-aminopropyltrimethoxysilane and glutaraldehyde as crosslinking agents.The color of ELISA products and the results of UV spectrophotometer showed that the adsorption ability of the modified glass to the analytes was improved greatly,and the problem of modification-recovery-remodification was solved.Finally,the surface functional groups before and after glass modification were analyzed by X-ray photoelectron spectroscopy.
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基金资助
国家自然科学基金(61501317);山西省自然科学基金(201701D221113);山西省高校科技创新研究项目(2016141)
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