以葡萄糖为碳源,采用一步超声法制备氮掺杂碳量子点,并进行海拉(Hela)细胞成像研究。所获得的氮掺杂碳量子点平均粒径3.3nm,并具有良好的水溶性、光致发光特性。氮掺杂碳量子点具有低细胞毒性,在氮掺杂碳量子点浓度为1mg/mL条件下,Hela细胞的存活率高于90%,在浓度高达5mg/mL条件下,Hela细胞的存活率仍高于60%。
Nitrogen-doped carbon dots (CDs) were synthesized directly by one-step ultrasonic treatment under mild conditions.And cell imaging experiments were carried out with HeLa cells.The results showed that the average size of CDs was approximately 3.3nm with good water solubility and photoluminescence.CDs had low toxicity,the results showed that more than 90% of Hela cells were viable when incubated with 1mg/mL or lesser CDs.In adittion,the cell viability was more than about 60% even at CDs concentration as high as 5mg/mL.
[1] Baker S N,Baker G A.Luminescent carbon nanodots:emergent nanolights[J].Angew Chem Int Ed,2010,49(38):6726-6744.
[2] Xu Xiaoyou,Ray Robert,Gu Yunlong,et al.Electrophoretic analysis and purification of fluorescent single-walled carbon nanotube fragments[J].J Am Chem Soc,2004,126(40):12736-12737.
[3] Zhou J G,Booker C,Li R Y,et al.An electrochemical avenue to blue luminescent nanocrystals from multiwalled carbon nanotubes[J].J Am Chem Soc,2007,129(4):744-745.
[4] Qiao Z A,Wang Y F,Gao Y,et al.Commercially activated carbon as the source for producing multicolor photoluminescent carbon dots by chemical oxidation[J].Chem Commun,2010,46(46):8812-8814.
[5] Zheng M,Xie Z G,Qu D,et al.On-off-on fluorescent carbon dot nanosensor for recognition of chromium (Ⅵ) and ascorbic acid based on the inner filter effect[J].ACS Appl Mater Interfaces,2013(5):13242-13247.
[6] Shen L M,Zhang L P,Chen M L,et al.The production of pH-sensitive photoluminescent carbon nanoparticles by the carbonization of polyethylenimine and their use for bioimaging[J].Carbon,2013,55:343-349.
[7] Guo X,Wang C F,Yu Z Y,et al.Facile access to versatile fluorescent carbon dots toward light-emitting diodes[J].Chem Commun,2012,48(21):2692-2694.
[8] Li H,He X,Liu Y,et al.One-step ultrasonic synthesis of water-soluble carbon nanoparticles with excellent photoluminescent properties[J].Carbon,2011,49(2):605-609.
[9] 初丛波,单玉萍,王宏达.量子点在生物检测中的应用[J].应用化学,2014,31(4):377-388.
[10] 梁梓承.生物质碳量子点的制备与应用研究[D].广州:华南理工大学,2016.
[11] Corneliu Sergiu Stan,Petronela Gospei Horlescu,Laura Elena Ursu,et a1.Facile preparation of highly luminescent composites by polymer embedding of carbon dots derived from N-hydroxyphthalimide[J].J Mater Sci,2017,52(1):185-196.
[12] Wang W J,Hai X,Mao Q X,et al.Polyhedral oligomeric silsesquioxane functionalized carbon dots for cell imaging[J].ACS Appl Mater Interfaces,2015,7(30):16609-16616.
[13] Bourlinos A B,Stassinopoulos A,Anglos D,et al,Surface functionalized carbogenic quantum dots[J].Small,2008,4(4):455-458.
[14] Li H,He X,Kang Z,et al.Water-soluble fluorescent carbon quantum dots and photocatalyst design[J].Angew Chem Int Ed,2010,49(26):4430-4434.
[15] Kim S,Hwang S W,Kim M K,et al.Anomalous behaviors of visible luminescence from graphene quantum dots:interplay between size and shape[J].ACS Nano,2012,6(9):8203-8208.
[16] Jin S H,Kim D A H,Jun G H,et al.Tuning the photoluminescence of graphene quantum dots through the charge transfer effect of functional groups[J].ACS Nano,2013,7(2):1239-1245.
[17] Sun X M,Li Y D.Colloidal carbon spheres and their core/shell structures with noble-metal nanoparticles[J].Aqngew Chem Int,Ed 2004,43(5):597-601.
[18] Wang L,Zhu S J,Wang H Y,et al.Common origin of green luminescence in carbon nanodots and graphene quantum dots[J].ACS Nano,2014,8(3):2541-2547.
基金资助
国家自然科学基金项目(51203015)
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