A toluidine blue (TB) molecularly imprinted polymer was prepared by solution polymerization with toluidine blue as template molecule,acrylamide as functional monomer,N,N′-methylene bisacrylamide as crosslinking agent and azodiisobutyronitrile as initiator.Then,the polymer was attached to the surface of chitosan-modified gold electrode by self-assembly method to prepare a TB molecularly imprinted electrochemical sensor.The electrochemical performance of the sensor was characterized by differential pulse voltammetry (DPV),cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS),and the surface of the sensor film was characterized by scanning electron microscope.The molar ratio of template molecules to functional monomers and the influence factors of elution time were optimized.The experimental results showed that the sensor had a good linear relationship with TB in the concentration range of 0～40.0×10-10g/L,the linear equation was y=0.0031x+0.0057,R2=0.9903,and its detection limit was 4.45×10-9g/L,The sensor had good selectivity,reproducibility and stability.This method was applied to the determination of TB in water samples,achieving a recovery rate of 102%.
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基金资助
福建省自然科学基金(2017J01421;2022J011160;2022J011164);福建省科技厅引导性项目(2020Y0089);福建省科学技术协会福建省科技经济融合服务平台(FJKX-2024XRH08);莆田市科技特派员项目(P2023KTP003);福建省科技特派员项目(F2024KTP015);莆田学院教育教学改革研究项目(JG202406)
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