以木质纤维素生物质的再生利用为背景,聚焦于一类小众但独到的纤维素体降解策略,重点介绍了纤维素体的结构组成和作用机理,归纳了改善纤维素体结构稳定性和催化活性的优化工程,并指出了纤维素体在工业应用中面临的挑战。
This review took the regeneration and utilization of lignocellulosic biomass as the background,focused on a niche but unique cellulosomal degradation strategy,and highlighted the structure composition and mechanism of cellulosomes.The optimization project to improve the stability and catalytic activity of cellulosomes was summarized,and the challenges of cellulosomes in industrial applications were pointed out.
[1] Deng W,Feng Y,Fu J,et al.Catalytic conversion of lignocellulosic biomass into chemicals and fuels[J].Green Energy & Environment,2023,8(1):10-114.
[2] Yadav V G,Yadav G D,Patankar S C.The production of fuels and chemicals in the new world:critical analysis of the choice between crude oil and biomass vis-à-vis sustainability and the environment[J].Clean Technologies and Environmental Policy,2020,22:1757-1774.
[3] Abolore R S,Jaiswal S,Jaiswal A K.Green and sustainable pretreatment methods for cellulose extraction from lignocellulosic biomass and its applications:a review[J].Carbohydrate Polymer Technologies and Applications,2024,7:100396.
[4] He Z J,Chen K,Liu Z H,et al.Valorizing renewable cellulose from lignocellulosic biomass toward functional products[J].Journal of Cleaner Production,2023,414:137708.
[5] Béguin P,Aubert J P.The biological degradation of cellulose[J].FEMS Microbiology Reviews,1994,13(1):25-58.
[6] Xu C,Tong S,Sun L,et al.Cellulase immobilization to enhance enzymatic hydrolysis of lignocellulosic biomass:an all-inclusive review[J].Carbohydrate Polymers,2023,321:121319.
[7] Reis C E R,Junior N L,Bento H B S,et al.Process strategies to reduce cellulase enzyme loading for renewable sugar production in biorefineries[J].Chemical Engineering Journal,2023,451:138690.
[8] Lamed R,Bayer E A.The cellulosome of clostridium thermocellum[J].Advances in Applied Microbiology,1988,33:1-46.
[9] Bayer E A,Morag E,Lamed R.The cellulosome—a treasure-trove for biotechnology[J].Trends in Biotechnology,1994,12(9):379-386.
[10] Lamed R,Setter E V A,Bayer E A.Characterization of a cellulose-binding,cellulase-containing complex in clostridium thermocellum[J].Journal of Bacteriology,1983,156(2):828-836.
[11] Moraïs S,Stern J,Artzi L,et al.Carbohydrate depolymerization by intricate cellulosomal systems[M]//Carbohydrate-Protein Interactions:Methods and Protocols.New York:Springer US,2023:53-77.
[12] Du C,Hu P,Ren L.Nucleic acid-based scaffold systems and application in enzyme cascade catalysis[J].Applied Microbio-logy and Biotechnology,2023,107(1):9-23.
[13] Wang X,Jiang Y,Liu H,et al.Research progress of multi-enzyme complexes based on the design of scaffold protein[J].Bioresources and Bioprocessing,2023,10(1):72.
[14] Lamote B,da Fonseca M J M,Vanderstraeten J,et al.Current challenges in designer cellulosome engineering[J].Applied Microbiology and Biotechnology,2023,107(9):2755-2770.
[15] Artzi L,Bayer E A,Moraïs S.Cellulosomes:bacterial nanomachines for dismantling plant polysaccharides[J].Nature Reviews Microbiology,2017,15(2):83-95.
[16] Andrade V B,Tomazetto G,Almeida D V,et al.Enzymatic and biophysical characterization of a novel modular cellulosomal GH5 endoglucanase multifunctional from the anaerobic gut fungus Piromyces finnis[J].Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics,2024,1872(1):140963.
[17] Duarte M,Alves V D,Correia M,et al.Structure-function studies can improve binding affinity of cohesin-dockerin interactions for multi-protein assemblies[J].International Journal of Biological Macromolecules,2023,224:55-67.
[18] Hamann P R V,Noronha E F.Xylan-breakdown apparatus of clostridium thermocellum[J].Cellulose,2022,29(14):7535-7553.
[19] Gilmore S P,Lillington S P,Haitjema C H,et al.Designing chimeric enzymes inspired by fungal cellulosomes[J].Synthe-tic and Systems Biotechnology,2020,5(1):23-32.
[20] Vanderstraeten J,da Fonseca M J M,De Groote P,et al.Combinatorial assembly and optimisation of designer cellulosomes:a galactomannan case study[J].Biotechnology for Biofuels and Bioproducts,2022,15(1):1-22.
[21] Tsai S L,Sun Q,Chen W.Advances in consolidated bioprocessing using synthetic cellulosomes[J].Current Opinion in Biotechnology,2022,78:102840.
[22] Doi R H,Kosugi A.Cellulosomes:plant-cell-wall-degrading enzyme complexes[J].Nature Reviews Microbiology,2004,2(7):541-551.
[23] Hirano K,Kurosaki M,Nihei S,et al.Enzymatic diversity of the clostridium thermocellum cellulosome is crucial for the degradation of crystalline cellulose and plant biomass[J].Scientific Reports,2016,6(1):35709.
[24] Gilbert H J.Cellulosomes:microbial nanomachines that display plasticity in quaternary structure[J].Molecular Microbiology,2007,63(6):1568-1576.
[25] Ding S Y,Bayer E A.Understanding cellulosome interaction with cellulose by high-resolution imaging[J].ACS Central Science,2020,6(7):1034-1036.
[26] Eibinger M,Ganner T,Plank H,et al.A biological nanomachine at work:watching the cellulosome degrade crystalline cellulose[J].ACS Central Science,2020,6(5):739-746.
[27] 杜济良,祁琪,刘涵,等.复合功能嵌合纤维小体空间结构优化及性能研究[J].太阳能学报,2022,43(6):246-250.
[28] Levi Hevroni B,Moraïs S,Ben-David Y,et al.Minimalistic cellulosome of the butanologenic bacterium clostridium saccharoperbutylacetonicum[J].MBio,2020,11(2):10.1128.
[29] Dorival J,Moraïs S,Labourel A,et al.Mapping the deformability of natural and designed cellulosomes in solution[J].Biotechnology for Biofuels and Bioproducts,2022,15(1):68.
[30] Wang Z,Yang C,Xue L,et al.Improving the efficiency of the assembly of cellulosomes derived from clostridium thermocellum by in Silico Design of Docking Protein[J/OL].Biotechnology Letters,2022.[2024-08-23].https://www.researchsquare.com/article/rs-1349924/v1.
[31] Anandharaj M,Lin Y J,Rani R P,et al.Constructing a yeast to express the largest cellulosome complex on the cell surface[J].Proceedings of the National Academy of Sciences,2020,117(5):2385-2394.
[32] Yadav A,Sharma V,Tsai M L,et al.Development of lignocellulosic biorefineries for the sustainable production of biofuels:towards circular bioeconomy[J].Bioresource Technology,2023,381:129145.
[33] Periyasamy S,Isabel J B,Kavitha S,et al.Recent advances in consolidated bioprocessing for conversion of lignocellulosic biomass into bioethanol-a review[J].Chemical Engineering Journal,2023,453:139783.
[34] Chen N,Yang S,You D,et al.Systematic genetic modifications of cell wall biosynthesis enhanced the secretion and surface-display of polysaccharide degrading enzymes in saccharomyces cerevisiae[J].Metabolic Engineering,2023,77:273-282.
[35] Fuchs W,Rachbauer L,Rittmann S K M R,et al.Eight up-coming biotech tools to combat climate crisis[J].Microorga-nisms,2023,11(6):1514.
[36] Lamote B,Vanderstraeten J,de Ridder N,et al.Customizing designer cellulosomes for the valorization of industrially relevant substrates[J/OL].SSRN,2024.[2024-08-26].https://www.researchsquare.com/article/rs-4750986/v1.
[37] Ye Y,Liu H,Wang Z,et al.A cellulosomal yeast reaction system of lignin-degrading enzymes for cellulosic ethanol fermentation[J].Biotechnology Letters,2024,46:531-543.
[38] 冯银刚,刘亚君,崔球.纤维小体在合成生物学中的应用研究进展[J].合成生物学,2022,3(1):138-154.
[39] Xiao M,Liu Y J,Bayer E A,et al.Cellulosomal hemicellulases:indispensable players for ensuring effective lignocellulose bioconversion[J].Green Carbon,2024,2(1):57-69.
[40] Madhavan A,Arun K B,Sindhu R,et al.Design and genome engineering of microbial cell factories for efficient conversion of lignocellulose to fuel[J].Bioresource Technology,2023,370:128555.
基金资助
青岛市源头创新科技计划项目(19-6-2-38-cg)
PDF
