通过一步水热法以柠檬酸和聚赖氨酸制备了碳化聚合物点,通过红外光谱仪、透射电子显微镜、X射线衍射对其形貌和组成进行表征;利用紫外-可见分光光度计和荧光分光光度计对碳化聚合物点的荧光特性进行测试;采用生长曲线法检测其对细菌生长的影响。结果表明:碳化聚合物点在365nm处可观察到蓝色荧光;其可选择性吸附作为生物生长必要元素之一的Fe3+,可用于三价铁的定量检测,检出限为3.4318μmol/L,检测范围是0～100μmol/L。碳化聚合物点对革兰阳性菌(金黄色葡萄球菌,S.aureus)和革兰阴性菌(大肠杆菌,E.coli)的最小抑菌浓度(MIC)分别是500μg/mL和250μg/mL。
Carbonized polymer dots were prepared via a one-step hydrothermal method from citric acid and polylysine.Their morphology and composition were characterized using infrared spectroscopy,transmission electron microscopy,and X-ray diffraction.The fluorescence properties of the carbonized polymer dots were tested using a UV-Vis spectrophotometer and a fluorescence spectrophotometer.The effect of the carbonized polymer dots on bacterial growth was assessed using the growth curve method.The results showed that the carbonized polymer dots exhibited blue fluorescence under 365nm light.They selectively adsorbed Fe3+,an essential element for biological growth,and could be used for the quantitative detection of trivalent iron with a detection limit of 3.4318μmol/L and a detection range of 0～100μmol/L.The minimum inhibitory concentrations (MIC) of the carbonized polymer dots against Gram-positive bacteria (Staphylococcus aureus,S.aureus) and Gram-negative bacteria (Escherichia coli,E.coli) were 500μg/mL and 250μg/mL,respectively.
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基金资助
常州市科技计划项目(CJ20220076);江苏理工学院研究生实践创新计划项目(XSJCX24-91)
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