硅橡胶被广泛应用于医疗器械领域,但植入人体后的硅橡胶表面容易吸附细菌,引起感染,增加了病人死亡的风险。为解决该问题,采用等离子体对硅橡胶进行表面改性,并与聚多巴胺(PDA)发生接枝反应,形成薄膜;然后通过化学镀法在其表面制备银抗菌涂层。通过原子力显微镜(AFM)、静态接触角测量仪等方法对样品表面进行分析;依据美国材料协会标准ASTM D3359-23表征涂层与硅橡胶之间的结合强度;采用抑菌环、活死细菌荧光染色法、酶标仪检测样品的抗菌性能及抗菌缓释性;运用电感耦合等离子原子发射发射光谱仪(ICP-AES)检测样品析出Ag+的浓度。结果表明:硅橡胶经等离子体改性后,提高了表面粗糙度,增强了薄膜与基体之间结合力,其结合力等级达到了3B。在硅橡胶表面形成的PDA薄膜,提升了硅橡胶表面的亲水性。PDA与抗菌涂层中Ag+的螯合,使样品具有较好的抗菌缓释性能及较长的抗菌寿命,25d后仍表现出良好的抗菌性。
Silicone rubber is widely used in the field of medical devices,but the surface of silicone rubber implanted into the human body is prone to absorb bacteria,which can cause infections,and increase the risk of patient mortality.In order to solve the problem,the surface of silicone rubber was modified by plasma,and the film was formed by grafting reaction with polydopamine.Then the silver antibacterial coating was prepared on the surface by electroless plating method.The surfaces of the samples were analyzed by atomic force microscope (AFM) and static contact angle measuring instrument.The bonding strength between the coating and silicone rubber was characterized according to the ASTM D3359-23 atandard of the American Society for Testing and Materials.The antibacterial properties and slow release properties of the samples were detected by inhibition zone test,fluorescent staining of live and dead bacteria and enzyme-labeled apparatus.The concentration of Ag+ was detected by inductively coupled plasma atomic emission spectrometer (ICP-AES).The results showed that the surface roughness of silicone rubber was improved by plasma modification,and the bonding force between the film and the matrix was enhanced,with a bonding grade of 3B.The PDA film formed on the silicone rubber surface improved the hydrophilicity of the silicone rubber surface.The chelation between PDA and Ag+ in the antibacterial coating made the sample have good antibacterial and slow release performance and long antibacterial lifetime,maintaining good antibacterial activity after 25d.
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基金资助
广西高校中青年教师科研基础能力提升项目(2022KY0795);国家自然科学基金地区项目(52361004)
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