化工新型材料

化工新型材料 ›› 2025, Vol. 53 ›› Issue (2) : 227-231. DOI: 10.19817/j.cnki.issn1006-3536.2025.02.032

开发与应用

镍基水凝胶的制备及传感性能的研究

^1*

作者信息 +

Study on the preparation and sensing properties of nickel-based hydrogel

Fan Lilin¹, Wang Ruotong¹, Pan Yuran¹, Cao Zheng¹, Cheng Junfeng¹, Liu Chunlin²

Author information +

文章历史 +

Received
2024-01-15
Issue Date	Revised Date
2025-02-20	2024-01-15

PDF

摘要

通过溶液热法将氢氧化镍(Ni(OH)₂)引入,采用自由基原位聚合法制备成多孔结构和高比表面积的聚丙烯酰胺/3-丙烯酰胺基苯硼酸@-氧化镍[PAM/3-APB@Ni(OH)₂水凝胶]。对水凝胶进行一系列测试表征,分析水凝胶的化学组成与微观形貌以及比色传感性能。结果表明:该水凝胶在酸性条件下分解过氧化氢(H₂O₂)生成羟基自由基,生成的羟基自由基进一步将无色的3,3′,5,5′-四甲基联苯胺(TMB)氧化为蓝色的氧化TMB(ox-TMB),在紫外光652nm处表现出明显的吸收值。该生物传感器对H₂O₂的检测范围为0～800μmol/L,且在30min内,含量20mg,pH=4且反应温度40℃时实现了非常低的检测限(LOD)(约3.34nmol/L),在H₂O₂检测中显示出巨大的潜力。

Abstract

Nickel hydroxide (Ni(OH)₂) was introduced through the solution thermal method,and the free radical in-situ polymerization method was used to prepare PAM/3-APB@Ni(OH)₂ hydrogel with porous structure and high specific surface area.A series of tests were performed to characterize the hydrogel and analyze the chemical composition and micromorphology of the hydrogel as well as its colorimetric sensing performance.Experimental results showed that the hydrogel decomposed hydrogen peroxide under acidic conditions to generate hydroxyl radicals,and the generated hydroxyl radicals further oxidized colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (ox-TMB),which exhibited obvious absorption value at 652nm in ultraviolet light.The detection range of this biosensor for hydrogen peroxide was 0～800μmol/L,and achieved a very low limit of detection (LOD) (about 3.34nmol/L) within 30 minutes at a content of 20mg,pH=4 and a reaction temperature of 40℃,demonstrating great potential in hydrogen detection.

关键词

丙烯酰胺 / 3-丙烯酰胺基苯硼酸 / 氢氧化镍 / 过氧化氢 / 比色传感

Key words

acrylamide / 3-acrylamidophenylboronic acid / nickel hydroxide / hydrogen peroxide / colorimetric sensing

引用本文

引用格式 ▾

镍基水凝胶的制备及传感性能的研究[J]. 化工新型材料, 2025, 53(2): 227-231 DOI:10.19817/j.cnki.issn1006-3536.2025.02.032

登录浏览全文

4963

注册一个新账户忘记密码

参考文献

原文顺序 | 出版日期 | 本文引用

[1] Nasri Z,Bruno G,Bekeschus S,et al.Development of an electrochemical sensor for in-situ monitoring of reactive species produced by cold physical plasma[J].Sensors and Actuators B:Chemical,2021,326:129007.
[2] Fort C I,Rusu M M,Cotet L C,et al.Carbon xerogel nanostructures with integrated Bi and Fe components for hydrogen peroxide and heavy metal detection[J].Molecules,2021,26:117.
[3] Zhu X,Chen W,Wu K,et al.A colorimetric sensor of H₂O₂ based on Co₃O₄-montmorillonite nanocomposites with peroxidase activity[J].New Journal of Chemistry,2018,42(2):1501-1509.
[4] Huang D,Gao S,Luo Y,et al.Glucose-sensitive membrane with phenylboronic acid-based contraction-type microgels as chemical valves[J].Journal of Membrane Science,2022,650:120406.
[5] Hennink W E,Van Nostrum C F.Novel crosslinking methods to design hydrogels[J].Advanced Drug Delivery Reviews,2012,64:223-236.
[6] Bapat A P,Roy D,Ray J G,et al.Dynamic-covalent macromolecular stars with boronic ester linkages[J].Journal of the American Chemical Society,2011,133(49):19832-19838.
[7] Ran Y,Moursy M,Hider R C,et al.The colorimetric detection of the hydroxyl radical[J].International Journal of Molecular Sciences,2023,24(4):4162.
[8] Zandieh M,Liu J.Surface science of nanozymes and defining a nanozyme unit[J].Langmuir,2022,38(12):3617-3622.
[9] Zeng J,Zhao B,Luo X,et al.Glucose-sensitive colorimetric sensor based on peroxidase mimics activity of carbon dots-functionalized Fe₃O₄ nanocomposites[J].Diamond and Related Materials,2023,136:109914.
[10] Zhang R,Yan X,Fan K.Nanozymes inspired by natural enzymes[J].Accounts of Materials Research,2021,2(7):534-547.
[11] Zuo L,Ren K,Guo X,et al.Amalgamation of DNAzymes and nanozymes in a coronazyme[J].Journal of the American Chemical Society,2023,145(10):5750-5758.
[12] Wu Y,Chen W,Wang C,et al.Overview of nanozymes with phosphatase-like activity[J].Biosensors and Bioelectronics,2023,237:115470.
[13] Song Y,Cho D,Venkateswarlu S,et al.Systematic study on preparation of copper nanoparticle embedded porous carbon by carbonization of metal-organic framework for enzymatic glucose sensor[J].RSC Advances,2017,7(17):10592-10600.
[14] Liu W,Tian L,Du J,et al.Triggered peroxidase-like activity of Au decorated carbon dots for colorimetric monitoring of Hg²⁺ enrichment in chlorella vulgaris[J].The Analyst,2020,145(16):5500-5507.
[15] 钟坚海,陈靖,黄建立,等.以硼和苯基共掺杂的石墨相氮化碳纳米片为模拟酶的过氧化氢荧光传感器[J].分析测试学报,2019,38(3):350-354.
[16] Su R,Xia L,Li G,et al.A controlled recognizing and releasing glycoprotein based on temperature-responsive phenylboronic microgels for colorimetric analysis of complex samples[J].Talanta,2022,241:123260.
[17] Siripongpreda T,Somchob B,Rodthongkum N,et al.Bacterial cellulose-based re-swellable hydrogel:facile preparation and its potential application as colorimetric sensor of sweat pH and glucose[J].Carbohydrate Polymers,2021,256:117506.
[18] Zhong N,Gao R,Shen Y,et al.Enzymes-encapsulated defective metal-organic framework hydrogel coupling with a smartphone for a portable glucose biosensor[J].Analytical Chemistry,2022,94(41):14385-14393.
[19] 罗自力,黄蓓,曹峥,等.基于PAM/CS/MoS₂复合水凝胶的QCM湿度传感器制备及应用[J].功能高分子学报,2023,36(1):86-94.
[20] 申艳敏,张鑫洋,孙晨,等.蒙脱石/壳聚糖/丙烯酸/丙烯酰胺四元共聚水凝胶的制备及吸附特性[J].化工新型材料,2023,51(2):172-176.
[21] Ni P,Sun Y,Dai H,et al.Prussian blue nanocubes peroxidase mimetic-based colorimetric assay for screening acetylcholinesterase activity and its inhibitor[J].Sensors and Actuators B:Chemical,2017,240:1314-1320.
[22] Wei H,Wang E.Fe₃O₄ Magnetic nanoparticles as peroxidase mimetics and their applications in H₂O₂ and glucose detection[J].Analytical Chemistry,2008,80(6):2250-2254.
[23] Ju Y,Kim J.Dendrimer-encapsulated Pt nanoparticles with peroxidase-mimetic activity as biocatalytic labels for sensitive colorimetric analyses[J].Chemical Communications,2015,51(72):13752-13755.
[24] Yu J,Ma D,Mei L,et al.Peroxidase-like activity of MoS₂ nanoflakes with different modifications and their application for H₂O₂ and glucose detection[J].Journal of Materials Chemistry B,2018,6(3):487-498.