以聚乙烯醇和壳聚糖为原料,利用冻融循环法,通过调节聚乙烯醇和壳聚糖的配比、冻融循环次数以及搅拌时间,采用正交试验制备出多组复合水凝胶,进行综合性能评价。结果表明:采用15%聚乙烯醇+1.5%壳聚糖混合搅拌4h,冻融循环5次制备的复合水凝胶具有较强的抗拉强度,适宜的含水率和溶胀率以及良好的血液相容性,对细胞无毒性且适合细胞生长,是一种具有应用前景的软骨修复组织工程材料。
Using polyvinyl alcohol and chitosan as raw materials,by employing a freeze-thaw cycle method and adjusting the ratio of polyvinyl alcohol and chitosan,the number of freeze-thaw cycles and the stirring time,a series of composite hydrogels were prepared by orthogonal experimental design.The comprehensive performance evaluation was conducted.The results showed that the composite hydrogel prepared by mixing and stirring 15wt.% polyvinyl alcohol and 1.5wt.% chitosan for 4 hours,and undergoing 5 freeze-thaw cycles had strong tensile strength,appropriate water content and swelling rate,good blood compatibility,non-toxicity to cells,and was suitable for cell growth,making it a promising tissue engineering material for cartilage repair.
[1] Li H J,Hu C,Yu H J,et al.Chitosan composite scaffolds for articular cartilage defect repair:a review[J].RSC Advances,2018,8(7):3736-3749.
[2] Oliveira A S,Silva J C,Loureiro M V,et al.Super-strong hydrogel composites reinforced with PBO nanofibers for cartilage replacement[J].Macromolecular Bioscience,2023,23(2):2200240.
[3] Wang S,Li H,Qi Z M,et al.Effects of freezing-thawing cycles on mechanical strength of poly (vinyl alcohol) hydrogels[C].Porto,Portugal:Proceedings of the 13th International Conference on Damage Assessment of Structures:DAMAS 2019,2020.
[4] Li H,Wu C W,Yu X G,et al.Recent advances of PVA-based hydrogels in cartilage repair application[J].Journal of Materials Research and Technology,2023,24:2279-2298.
[5] Wei W,Ma Y Z,Yao X D,et al.Advanced hydrogels for the repair of cartilage defects and regeneration[J].Bioactive Materials,2021,6(4):998-1011.
[6] Bai X,Lü S Y,Cao Z,et al.Self-reinforcing injectable hydrogel with both high water content and mechanical strength for bone repair[J].Chemical Engineering Journal,2016,288:546-556.
[7] Wu Y Y,Li X L,Wang Y F,et al.Research progress on mechanical properties and wear resistance of cartilage repair hydrogel[J].Materials & Design,2022,216:110575.
[8] 张凡,胡钦南,宋玲玲,等.可拉伸、自修复Janus水凝胶薄膜用于柔性传感器的研究[J].现代化工,2023,43(10):160-166,173.
[9] Li W X,Wang D,Yang W,et al.Compressive mechanical properties and microstructure of PVA-HA hydrogels for cartilage repair[J].RSC Advances,2016,6(24):20166-20172.
[10] 林博偲,王会,李久盛,等.掺锶复合PVA/生物活性玻璃水凝胶的制备及其体外软骨修复性能研究[J].生物骨科材料与临床研究,2020,17(4):7-11,20.
[11] Takamura A,Arai M,Ishii F.Drug release from freeze-thaw poly (vinyl alcohol) gel[J].Journal of the Pharmaceutical Society of Japan,1987,107(3):233-237.
[12] 李一凡,刘捷,李政雄.PVA水凝胶制备,改性及在生物医学工程中的研究进展[J].硅谷,2012(7):5-6.
[13] Ng K W,Torzilli P A,Warren R F,et al.Characterization of a macroporous polyvinyl alcohol scaffold for the repair of focal articular cartilage defects[J].Journal of Tissue Engineering and Regenerative Medicine,2014,8(2):164-168.
[14] Gupta S,Goswami S,Sinha A.A combined effect of freeze—thaw cycles and polymer concentration on the structure and mechanical properties of transparent PVA gels[J].Biomedical Materials,2012,7(1):015006.
[15] Zhang K,Liu Y,Shi X W,et al.Application of polyvinyl alcohol/chitosan copolymer hydrogels in biomedicine:a review[J].International Journal of Biological Macromolecules,2023:125192.
[16] Stauffer S R,Peppast N A.Poly (vinyl alcohol) hydrogels prepared by freezing-thawing cyclic processing[J].Polymer,1992,33(18):3932-3936.
[17] 杨迪.基于壳聚糖季铵盐的抗菌水凝胶的制备及性能研究[D].长春:长春工业大学,2023.
[18] Ricciardi R,Auriemma F,De Rosa C,et al.X-ray diffraction analysis of poly (vinyl alcohol) hydrogels,obtained by freezing and thawing techniques[J].Macromolecules,2004,37(5):1921-1927.
[19] Ricciardi R,Auriemma F,Gaillet C,et al.Investigation of the crystallinity of freeze/thaw poly (vinyl alcohol) hydrogels by different techniques[J].Macromolecules,2004,37(25):9510-9516.
[20] 李津铭.关节软骨替代用聚乙烯醇水凝胶的力学性能调控[D].大连:大连理工大学,2022.
[21] Sun Y J,Xiang N P,Jiang X C,et al.Preparation of high tough poly (vinyl alcohol) hydrogel by soaking in NaCl aqueous solution[J].Materials Letters,2017,194:34-37.
[22] Millon L E,Mohammadi H,Wan W K.Anisotropic polyvinyl alcohol hydrogel for cardiovascular applications[J].Journal of Biomedical Materials Research Part B Applied Biomaterials,2006,79(2):305-311.
[23] Zhao N N,Wu F,Xing Y,et al.A flexible hydrogel electrolyte with superior mechanical properties based on poly (vinyl alcohol) and bacterial cellulose for the solid-state zinc-air batteries[J].ACS Applied Materials & Interfaces,2019,11(17):15537-15542.
[24] Luo C H,Zhao Y F,Sun X X,et al.Fabrication of antiseptic,conductive and robust polyvinyl alcohol/chitosan composite hydrogels[J].Journal of Polymer Research,2020,27:1-9.
[25] Chen S C,Wu Y C,Mi F L,et al.A novel pH-sensitive hydrogel composed of N,O-carboxymethyl chitosan and alginate cross-linked by genipin for protein drug delivery[J].Journal of Controlled Release,2004,96(2):285-300.
[26] Berger J,Reist M,Mayer J M,et al.Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications[J].European Journal of Pharmaceutics and Biopharmaceutics,2004,57(1):35-52.
[27] Bi S C,Wang P J,Hu S H,et al.Construction of physical-crosslink chitosan/PVA double-network hydrogel with surface mineralization for bone repair[J].Carbohydrate Polymers,2019,224:115176.
[28] 陈阳,郭亚欣,杨明成,等.聚乙烯醇/壳聚糖水凝胶的辐射制备及其生物相容性研究[J].化工新型材料,2023,51(11):229-233.
[29] Wang T,Gunasekaran S.State of water in chitosan-PVA hydrogel[J].Journal of Applied Polymer Science,2006,101(5):3227-3232.
[30] 李扬帆.PVA基水凝胶的制备及磨损性能研究[D].太原:中北大学,2022.
[31] 吴麟.高性能PVA基复合水凝胶的制备及其性能研究[D].广州:暨南大学,2022.
基金资助
国家自然科学基金(52175286);北京石油化工学院国家级大学生创新创业训练计划项目(2024J00084和2019J00094);北京石油化工学院交叉科研探索项目(BIPTCSF-019);北京亦城合作发展基金会科研项目(YJXJ-JZ-2021-0014-11)
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