以葡萄酒残渣(GWR)为研究对象,测定了其活性成分的含量信息由高到低依次为:糖类、多酚、黄酮、蛋白质。用GWR实现了金纳米颗粒(GNPs)的绿色制备,通过紫外-可见分光光度计、傅里叶红外光谱、透射电子显微镜等技术手段对GNPs的结构形态进行表征。分析得到GWR中酚类或多羟基类物质作为还原剂存在,而起保护作用的主要为多酚和黄酮类物质,GNPs结构为面心立方晶体。通过控制变量探究了GNPs的合成规律,发现酸碱度和反应温度是影响GNPs形貌的主要因素,而提取液浓度与反应容器是影响GNPs粒径的主要因素。
Taking grape wine residue (GWR) as the research object,the content information of its active components was determined in the order from high to low:sugars,polyphenols,flavonoids and proteins.The green preparation of gold nanoparticles (GNPs) was realized by GWR.The structure and morphology of GNPs were characterized by UV-Vis spectrophotometer,Fourier infrared spectrum,and transmission electron microscope.The analysis showed that phenols or polyhydroxy substances in GWR existed as reducing agents,and the main protective substances were polyphenols and flavonoids.The structure of GNPs was a face-centered cubic crystal.The synthesis law of GNPs was explored by controlling variables,and it was found that pH and reaction temperature were the main factors affecting the morphology of GNPs,while the concentration of extraction solution and reaction vessel were the main factors affecting the particle size of GNPs.
[1] Ahmad N,Bhatnagar S,Saxena R,et al.Biosynthesis and characterization of gold nanoparticles:kinetics,in vitro and in vivo study[J].Materials Science & Engineering C-Materials,2017,78:553-564.
[2] Yildirim O,Ozkaya B.Effect of nanoparticles synthesized from green extracts on dark fermentative biohydrogen production[J].Biomass & Bioenergy,2023,170:106707.
[3] Merugu R,Sharma S,Rastogi M.Application of bimetallic Zr-Co nanoparticles synthesized by a green route for antibacterial,antioxidant and antitumor activities[J].Materials Today:Proceedings,2023,1:262.
[4] Mariychuk R,Smolkova R,Bartosova V,et al.The regularities of the Mentha piperita L.extract mediated synthesis of gold nanoparticles with a response in the infrared range[J].Applied Nanoscience,2021,12:1071-1083.
[5] Yang X,Wang W,Wang C,et al.Synthesis of Au decorated V2O5 microflowers with enhanced sensing properties towards amines[J].Powder Technology,2018,339:408-418.
[6] Choi D H,Hong J E,Jung Y,et al.Synthesis of gold nanoparticles supported at graphene derivatives using green reductants and evaluation of their catalytic activity in 4-nitrophenol reduction[J].Gold Bulletin,2019,52:165-174.
[7] Khan M,Ahmad F,Koivisto J T,et al.Green synthesis of controlled size gold and silver nanoparticles using antioxidant as capping and reducing agent[J].Colloid and Interface Science Communications,2020,39:100322.
[8] Olajire A A,Mohammed A A.Bio-directed synthesis of gold nanoparticles using Ananas comosus aqueous leaf extract and their photocatalytic activity for LDPE degradation[J].Advanced Powder Technology,2021,32:600-610.
[9] Muniyappan N,Pandeeswaran M,Amalraj A.Green synthesis of gold nanoparticles using Curcuma pseudomontana isolated curcumin:Its characterization,antimicrobial,antioxidant and anti-inflammatory activities[J].Environmental Chemistry and Ecotoxicology,2021,3:117-124.
[10] Li S,Al-Misned F A,El-Serehy H A,et al.Green synthesis of gold nanoparticles using aqueous extract of Mentha Longifolia leaf and investigation of its anti-human breast carcinoma properties in the in vitro condition[J].Arabian Journal of Chemistry,2021,14:102931.
[11] Singh A T,Shaikh I N,Rale M V.Synthesis of gold and silver nanoparticles using Phlogacanthus Thyrsiformis extracts-its phytochemical screening and anti-oxidant studies[J].Materials Today:Proceedings,2020,43:2805-2809.
[12] Zhang N,Hoadley A,Patel J,et al.Sustainable options for the utilization of solid residues from wine production[J].Waste Manage,2017,60:173-183.
[13] Aliakbarian B,Fathi A,Perego P,et al.Extraction of antioxidants from winery wastes using subcritical water[J].Journal of Supercritical Fluids,2012,65(65):18-24.
[14] 吴建华,吴志瑰,裴建国,等.多酚类化合物的研究进展[J].中国现代中药,2015,17(6):630-636.
[15] Wei Y,Bishop K J,Kim J,et al.Making use of bond strength and steric hindrance in nanoscale “synthesis”[J].Angewandte Chemie International Edition,2009,48(50):9477-9480.
[16] Sugikawa K,Matsuo K,Ikeda A.Suppression of gold nanoparticle aggregation on lipid membranes using nanosized liposomes to increase steric hindrance[J].Langmuir,2018,35(1):229-236.
[17] Islam M.T,Molugu S.K,Cooke P.H,et al.Fullerene stabilized gold nanoparticles[J].New Journal of Chemistry,2015,39(8):5923-5926.
[18] Zhang L,Han F.Protein coated gold nanoparticles as template for the directed synthesis of highly fluorescent gold nanoclusters[J].Nanotechnology,2018,29(16):165702.
[19] Verma N.A green synthetic approach for size tunable nanoporous gold nanoparticles and its glucose sensing application[J].Applied Surface Science,2018,462:753-759.
[20] Sekhar M C,Reddy B P,Mallikarjuna K,et al.Biogenic fabrication of Au/Pd bimetallic quantum dots from mushroom extract and their application to organic dye pollutant reduction[J].Current Nanoscience,2018,14(4):313-318.
[21] Banerjee S,Kumar N P,Srinivas A,et al.Core-shell Fe3O4@Au nanocomposite as dual-functional optical probe and potential removal system for arsenic(Ⅲ) from water[J].Journal of Hazardous Materials,2019,375:216-223.
基金资助
宁夏自然科学基金项目(2021AAC03200);北方民族大学中央高校基本科研业务费专项资金资助项目(2023XYZHG02)
PDF
