以间苯二酚甲醛树脂为前驱体,三聚氰胺为氮源,球形碳酸钙为模板剂,氢氧化钾为扩孔剂,通过硬模板法合成了氮掺杂中空碳球(NHCSs),通过扫描电子显微镜、场发射透射电子显微镜、X射线光电子能谱仪对 NHCSs的微观形貌、表面元素组成及化学价态进行了表征,探究了NHCSs过氧化物模拟酶活性及反应体系中产生的活性氧中间体。结果表明:在弱酸性和H2O2存在的条件下,NHCSs可以快速地催化氧化3,3′,5,5′-四甲基联苯胺(TMB)生成蓝色产物;经过氢氧化钾扩孔处理后的NHCS-1对TMB和H2O2具有良好的亲和力;超氧自由基·O-2和羟基自由基·OH是主要的活性物种。采用简单快速的比色法检测H2O2,线性范围为8～1000μmol/L,检测限为4.97μmol/L。
Using resorcinol formaldehyde resin as precursor,melamine as nitrogen source,spherical calcium carbonate (CaCO3) as template agent and KOH as pore expanding agent,N-doped hollow carbon spheres (NHCSs) were synthesized by hard template method.The microscopic morphology,surface element composition and chemical valence states of NHCSs were characterized by scanning electron microscope,field emission transmission electron microscope and X-ray photoelectron spectrometer,and the activity of NHCSs peroxide-simulated enzyme and reactive oxygen species produced in the reaction system were investigated.The results showed that NHCSs could rapidly catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue product under the condition of weak acidity and the presence of H2O2.NHCS-1 after KOH reaming had a good affinity for TMB and H2O2.The reactive oxygen intermediates produced in the reaction system were explored,and it was proved that ·O-2 and ·OH were the main active species.A simple and rapid colorimetric method was used to detect H2O2,with a linear range of 8～1000μmol/L.The detection limit was 4.97μmol/L.
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基金资助
国家自然科学基金(52162004);广西石化资源加工及过程强化技术重点实验室主任基金(2020Z003);广西博世科产学研合作项目(20210014)
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