开发了一种新型荧光传感器,即利用甲硫氨酸(Met)作为保护剂和还原剂,微波辅助快速合成了具有橙色荧光的金纳米簇(Met-AuNCs),用于检测水杨醛(SA)。在检测中,SA的醛基和AuNCs上的氨基发生亲核加成,形成席夫碱,与此同时Met的表面状态被改变,使AuNCs失去氨基酸配体的保护发生聚集。随着SA浓度的增加,AuNCs的特征荧光峰消失,而席夫碱的荧光强度逐渐增强,荧光由橙色变为绿色。用于SA检测的荧光传感器具有简单、快速、肉眼检测、高灵敏度等优点。该方法检测SA的线性范围为0.1～9.4μmol/L,检测限(LOD)低至7.4nmol/L。
A novel fluorescence sensor,gold nanoclusters (AuNCs) with orange fluorescence,for the detection of salicylaldehyde (SA) was developed by using methionine (Met) as a protecting and reducing agent and microwave-assisted rapid synthesis.In the detection,nucleophilic addition occurred between the aldehyde group of SA and the amino group of AuNCs,forming a Schiff base.Simultaneously the surface state of Met was altered so that the AuNCs lost the protection of amino acid ligands and aggregated.With the increase of SA concentration,the intrinsic fluorescence peak disappeared,while the fluorescence intensity of Schiff base gradually increased,and the fluorescence changed from orange to green.The fluorescence sensor for SA detection had advantages of simplicity,rapidity,naked-eye detection,and high sensitivity.The linear range of the method for SA detection was from 0.1μmol/L to 9.4μmol/L,with a low limit of detection (LOD) of 7.4nmol/L.
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基金资助
国家自然科学基金(21407035);山东省自然科学基金项目(ZR2014BM021)
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