化工新型材料

化工新型材料 ›› 2023, Vol. 51 ›› Issue (3) : 227-233. DOI: 10.19817/j.cnki.issn1006-3536.2023.03.040

科学研究

Ag/Fe₃O₄纳米复合材料的绿色合成及其抑菌机制

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Green synthesis and antibacterial mechanism of Ag/Fe₃O₄ nanocomposites

Su Tianhao, Liu Kangkang, Zhang Xiaodong, Long Xiujiao, Chen Saili, Cao Hailei, Lv Jian

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文章历史 +

Received		Published
2022-10-19		2023-03-20
Issue Date	Revised Date
2023-03-29	2022-10-19

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摘要

以茶叶提取液为还原剂,采用原位还原方法绿色合成Ag/Fe₃O₄复合材料,系统表征了复合材料的形貌及组成特性,并对其抑菌性能及机制进行了深入分析。X射线衍射(XRD)、X射线光电子能谱(XPS)表征结果表明:Ag(I)被还原为Ag⁰;振动样品磁强计(VSM)测试表明:Ag/Fe₃O₄具有较高的饱和磁化强度;透射电镜(TEM)测试表明:所负载Ag⁰粒径在50~100nm之间;红外光谱(FT-IR)测试结果表明:茶叶提取液中的酚羟基起到了桥接金属元素及还原剂的作用。复合材料的抑菌研究表明,Ag/Fe₃O₄对大肠杆菌和金黄色葡萄球菌的生长均有良好的抑制效果,其最低抑菌浓度(MIC)分别为1.76μg/mL和7.04μg/mL。通过碱性磷酸酶(AKP)、核酸、蛋白质的测定,以及扫描电镜(SEM)表征,证实Ag/Fe₃O₄的接触使菌体表面结构坍塌、内陷、甚至分裂,并通过影响菌体细胞壁、细胞膜的通透性,影响其基因的表达,达到抑菌目的。

Abstract

Ag/Fe₃O₄ nanocomposites were green synthesized by in situ reduction method using tea extract as the reducing agent,and then the morphology and compositions of the composites were systematically characterized.After that,their antibacterial properties and mechanisms were intensively studied.The reduction of Ag(I) to Ag⁰ on the Fe₃O₄ carrier was verified by X-ray powder diffraction and X-ray photoelectron spectroscopy.VSM test showed that Ag/Fe₃O₄ possessed high saturation magnetization intensity.The particle size of the synthesized Ag⁰ ranged from 50 to 100 nm,which demonstrated by transmission electron microscopy images.Infrared spectra exhibited that the phenolic hydroxyl groups in tea extract acted as reducing agent as well as a bridge that connecting Ag and Fe elements during the reduction process.The results of growth curve and antibacterial activity implied that Ag/Fe₃O₄ had a good inhibitory effect on the growth of Escherichia coli and Staphylococcus aureus.The minimum inhibitory concentration (MIC) of Ag/Fe₃O₄ composite against Escherichia coli and Staphylococcus aureus were 1.76μg/mL and 7.04μg/mL,respectively.The determination of alkaline phosphatase (AKP),nucleic acid and protein,and SEM characterization confirmed that the contact of Ag/Fe₃O₄ caused the collapse,invagination and even division of the cell surface structure,and affected the permeability of cell wall and cell membrane.Consequently,the expression of the genes was strongly affected to achieve the purpose of bacteriostasis.

关键词

绿色合成 / 银 / 磁性纳米复合材料 / 抑菌机制

Key words

green synthesis / silver / magnetic nanocomposites / bacteriostatic mechanism

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Ag/Fe₃O₄纳米复合材料的绿色合成及其抑菌机制[J]. 化工新型材料, 2023, 51(3): 227-233 DOI:10.19817/j.cnki.issn1006-3536.2023.03.040

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