开发与应用

聚己内酯/羟基磷灰石晶须复合多孔支架的研究

展开
  • 昆明理工大学材料科学与工程学院,昆明650093
李潘(1994-),男,硕士研究生,从事生物功能材料与高分子复合材料的研究。

收稿日期: 2019-10-24

  修回日期: 2020-12-06

  网络出版日期: 2021-03-03

基金资助

云南省大学生创新创业训练项目PCL/PVA复合人造皮肤的制备及应用基础研究(201810672002)

Fabrication and property of PCL/HAw composite porous scaffold

Expand
  • Faculty of Materials Science and Engineering,Kunming University of Science and Technology, Kunming 650093

Received date: 2019-10-24

  Revised date: 2020-12-06

  Online published: 2021-03-03

摘要

采用溶液浇铸法,以二氯甲烷作为溶剂,制备了聚己内酯/羟基磷灰石晶须(PCL/HAw)复合多孔支架,并进行了正交试验,综合分析了不同配方量的PCL和HAw对材料机械性能的影响。结果表明,可通过控制PCL的量来控制支架的力学性能,通过加入HAw提高支架的亲水性能,支架的接触角实验显示其接触角为81°;PCL的结晶度会随着HAw含量的增加而增强,复合多孔支架的抗拉强度为1.43M~9.21MPa,并在PCL与HAw的质量比为100∶3时达到最大;细胞毒性实验显示,PCL/HAw复合多孔支架细胞毒性为0,满足生物材料使用要求。

本文引用格式

李潘, 钟聚婧, 秦晓素, 颜廷亭 . 聚己内酯/羟基磷灰石晶须复合多孔支架的研究[J]. 化工新型材料, 2021 , 49(2) : 235 -239 . DOI: 10.19817/j.cnki.issn 1006-3536.2021.02.054

Abstract

Polycaprolactone (PCL)/hydroxyapatite whisker (HAw) composite porous scaffolds were prepared by solution casting method with dichloromethane as solvent.The orthogonal test was conducted to compare composites with the different formulas of PCL and HAw.The results shown that the mechanical properties of the scaffolds were controlled by the amount of PCL.The hydrophilicity of the scaffolds could be improved by adding HAw.The composites shown that the contact angle was 81°,which was hydrophilic.The crystallinity of PCL increased with the increasing of HAw.The tensile strength of the scaffolds was 1.43~9.21MPa,and reached to the maximum when the mass ratio of PCL to HAw was 100∶3.The cytotoxicity experiment showed that the cytotoxicity of the scaffolds was 0,which could meet the requirements for the use of biological materials.

参考文献

[1] Sherwood J K,Riley S L,Palazzolo R,et al.A three-dimensional osteochondral composite scaffold for articular cartilage repair[J].Biomaterials,2002,23(24):4739-4751.
[2] Hildebrand T,Rüegsegger P.Quantification of bone microarchitecture with the structure model index[J].Computer Methods in Biomechanics and Biomedical Engineering,1997,1(1):15-23.
[3] Bose S,Roy M,Bandyopadhyay A.Recent advances in bone tissue engineering scaffolds[J].Trends in Biotechnology,2012,30(10):546-554.
[4] Woodward S C,Brewer P S,Moatamed F,et al.The intracellular degradation of poly(ε-caprolactone)[J].Journal of Biomedical Materials Research,1985,19(4):437-444.
[5] Ulery B D,Nair L S,Laurencin C T.Biomedical applications of biodegradable polymers[J].Journal of Polymer Science Part B:Polymer Physics,2011,49(12):832-864.
[6] Ang K C,Leong K F,Chua C K,et al.Compressive properties and degradability of poly(epsilon-caprolatone)/hydroxyapatite composites under accelerated hydrolytic degradation[J].Journal of Biomedical Materials Research Part A,2007,80(3):655-660.
[7] Adhikari U,An X,Rijal N,et al.Embedding magnesium metallic particles in polycaprolactone nanofiber mesh improves applicability for biomedical applications[J].Acta Biomaterialia,2019,98:215-234.
[8] Shaltooki M,Dini G,Mehdikhani M.Fabrication of chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass nanocomposite scaffold for bone tissue engineering[J].Materials Science and Engineering:C,2019,105:110138.
[9] Gandolfi M G,Zamparini F,Degli Esposti M,et al.Highly porous polycaprolactone scaffolds doped with calcium silicate and dicalcium phosphate dihydrate designed for bone regeneration[J].Materials Science and Engineering:C,2019,102:341-361.
[10] Linhart W,Briem D,Amling M,et al.Mechanisches versagen einer porösen hydroxylapatitkeramik 7,5 jahre nach implantation an der proximalen Tibia[J].Der Unfallchirurg,2004,107(2):154-157.
[11] Fan X.Preparation and performance of hydroxyapatite/Ti porous biocomposite scaffolds[J].Ceramics International,2019,45(13):16466-16469.
[12] Matinfar M,Mesgar A S,Mohammadi Z.Evaluation of physicochemical,mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering[J].Materials Science and Engineering:C,2019,100:341-353.
[13] Converse G L,Conrad T L,Merrill C H,et al.Hydroxyapatite whisker-reinforced polyetherketoneketone bone ingrowth scaffolds[J].Acta Biomaterialia,2010,6(3):856-863.
[14] Matinfar M,Mesgar A S,Mohammadi Z.Evaluation of physicochemical,mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering[J].Materials Science and Engineering:C,2019,100:341-353.
[15] Lam C X F,Hutmacher D W,Schantz J,et al.Evaluation of polycaprolactone scaffold degradation for 6 months in vitro and in vivo[J].Journal of Biomedical Materials Research Part A,2009,90A(3):906-919.
[16] 徐艺展,何丹,肖秀峰,等.羟基磷灰石/聚己内酯生物复合材料的制备及其生物活性[J].华东理工大学学报(自然科学版),2006,32(6):709-713.
[17] Zhang J,Liu H,Ding J,et al.High-pressure compression-molded porous resorbable polymer/hydroxyapatite composite scaffold for cranial bone regeneration[J].ACS Biomaterials Science & Engineering,2016,2(9):1471-1482.
[18] 赵惠,李潇,金柱坤,等.影响种植支架材料生物相容性的因素[J].国际口腔医学杂志,2014,41(3):341-346.
[19] Ju J,Peng X,Huang K,et al.High-performance porous PLLA-based scaffolds for bone tissue engineering:preparation,characterization,and in vitro and in vivo evaluation[J].Polymer,2019,180:121707.
[20] 贺江平.PET/SiO2纳米复合材料的制备与性能研究[D].湘潭:湘潭大学,2007.
[21] 陈佳佳.纳米羟基磷灰石与聚己内酯复合电纺膜的制备及性能研究[D].合肥:安徽大学,2012.
[22] Guarino V,Taddei P,Foggia M D,et al.The influence of hydroxyapatite particles on in vitro degradation behavior of poly ε-caprolactone-based composite scaffolds[J].Tissue Engineering Part A,2009,15(11):3655-3668.
[23] Girard F,Antoni M,Sefiane K.On the effect of marangoni flow on evaporation rates of heated water drops[J].Langmuir:the ACS Journal of Surfaces and Colloids,2008,24:9207-9210.
[24] Rumpler M,Woesz A,W C Dunlop J,et al.The effect of geometry on three-dimensional tissue growth[J].Journal of the Royal Society,Interface/the Royal Society,2008,5:1173-1180.
[25] Kuboki Y,Jin Q,Kikuchi M,et al.Geometry of artificial ECM:sizes of pores controlling phenotype expression in BMP-induced osteogenesis and chondrogenesis[J].Connective Tissue Research,2002,43(2-3):529-534.
[26] Guarino V,Causa F,Netti P A,et al.The role of hydroxyapatite as solid signal on performance of PCL porous scaffolds for bone tissue regeneration[J].Journal of Biomedical Materials Research Part B,2008,85(2):524-531.
[27] Ang K C,Leong K F,Chua C K.Compressive properties and degradability of poly(8-caprolatone)/hydroxyapatite composites under accelerated hydrolytic degradation[J].Journal of Biomedical Materials Research Part A,2007,80A(3):278-290.
[28] Lin Z,Zhang X,Zhao F,et al.Bisphenol S promotes the cell cycle progression and cell proliferation through ERα-cyclin D-CDK4/6-pRb pathway in MCF-7 breast cancer cells[J].Toxicology and Applied Pharmacology,2019,366:75-82.
[29] Zhong X,Liu D,Hao Y,et al.The expression of TET3 regulated cell proliferation in HepG2 cells[J].Gene,2019,698:113-119.
[30] 张燕搏,姜明,王强,等.组织工程血管材料体外细胞毒性评价实验研究[J].中华实用诊断与治疗杂志,2011,25(6):539-542.
[31] 中国国家标准化管理委员会中华人民共和国国家质量监督检验检疫总局.医用输液、输血、注射器具检验方法第2部分:生物学试验方法:GB/T 14233.2—2005[Z].31.
Options
文章导航

/