为实现对低浓度H2O2的快速检测,以玻碳电极为基底,Nafion(NF)为分散剂、成膜剂,原位电化学还原氧化石墨烯(GO)、电化学聚合苯胺,得到还原氧化石墨烯/聚苯胺(ERGO/PANI)复合物,以ERGO/PANI为修饰物,构建了无酶电化学传感器PANI/ERGO-NF修饰电极(PANI/ERGO-NF/GCE),对H2O2进行检测。利用扫描电镜和拉曼光谱对修饰电极的表面形貌和分子结构进行表征;利用电化学阻抗谱和循环伏安法研究了修饰电极的电化学性能及对H2O2的响应性能。结果表明:PANI/ERGO-NF/GCE对H2O2的电化学氧化显示出较好的催化活性,具有较高的检测灵敏度、较宽的线性范围以及较好的重现性、稳定性和抗干扰能力,在线性范围1～500μmol/L内,检出限0.1μmol/L。
In order to achieve rapid detection of low concentration hydrogen peroxide (H2O2),a non-enzymatic H2O2 electrochemical sensor was constructed by using glassy carbon electrode as substrate,Nafion as selective coating material,electrochemical reduced graphene oxide and electrochemical polymerized polyaniline complex (ERGO/PANI)as modifier.The microstructure and molecular structure of the modified electrode was characterized by SEM and raman spectroscopy.The electrochemical properties of the modified electrode and its response to H2O2 were studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry.Results shown that the constructed PANI/ERGO-NF/GCE electrode exhibited excellent electrocatalytic performance for the determination of H2O2.The proposed sensor displayed a wide linear range of 1.00～5.00μmol/L,a limit of detection (LOD) of 0.1μmol/L.Moreover,the sensor shown outstanding stability,reproducibility and anti-interference ability.
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