采用石墨烯纳米材料,并结合葡萄糖氧化酶、聚苯胺和纳米银修饰玻碳电极研制了新型的生物传感器。考察了修饰电极的电化学行为,讨论了3种不同影响因素对电极响应的作用。结果表明:传感器对葡萄糖表现出良好的电催化性能。在最佳条件下,该传感器对葡萄糖的线性检测范围为5×10-6～3.2×10-4mol/L,检测限为6.65×10-6mol/L(S/N=3)。
A new glucose biosensor based on Ag nanoparticles,graphene,polyaniline (PANI) and glucose oxidase(GOD) modified electrode was prepared.The electrochemical behavior of the biosensor was investigated by UV-Vis spectra,electrochemical impedance spectroscopy and cyclic voltammetry method.The performances of the biosensor were observed by the applied potential,pH value and concentration of GOD.The results indicated that the biosensor possessed high catalytic activity.Under the optimal experimental conditions,the current was linear with the concentration of glucose in the range from 5×10-6 to 3.2×10-4mol/L,with a detection limit of 6.65×10-6mol/L (S/N=3) and a correlation coefficient of 0.998.
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