以盐酸多巴胺为单体,在三羟甲基氨基甲烷溶液中聚合,合成了聚多巴胺纳米球(PDANSs)。采用扫描电镜和透射电镜对PDANSs进行了形貌表征,PDANSs直径为200～300nm。将制备的PDANSs在十六烷基三甲基溴化铵(CTAB)溶液中超声,通过静电作用得到复合物(PDANSs/CTAB),并将复合物分散液滴涂在电极表面得到PDANSs/CTAB修饰电极。将PDANSs/CTAB修饰电极用于溶液中亚硝酸盐的富集,对电极上富集的亚硝酸盐含量用差分脉冲伏安法进行了测定,构建了亚硝酸盐电化学传感器。该传感器检测亚硝酸钠的线性范围为0.2～2000μmol/L,检出限为0.08μmol/L,且具有较好的重现性和稳定性。将该传感器用于腌制肉样品中亚硝酸盐的检测,回收率范围为98.1%～104.0%,说明此方法准确度很好,该传感器能用于腌制肉样品中亚硝酸盐的分析。
Polydopamine nanospheres (PDANSs) were synthesized by polymerization of dopamine hydrochloride as a monomer in tris(hydroxymethyl)aminometha solution.The morphology of PDANSs was characterized using scanning electron microscopy and transmission electron microscopies,revealing a diameter of 200～300nm.The prepared PDANSs were sonicated in a hexadecyltrimethylammonium bromide (CTAB) solution,and PDANSs/CTAB composite was obtained through the electrostatic interaction.The prepared composite dispersant was drop-coated on the electrode surface to obtain a PDANSs/CTAB-modified electrode.The PDANSs/CTAB-modified electrode was used to enrich nitrite in solution,and the content of nitrite enriched on the electrode was determined by differential pulse voltammetry,and a nitrite electrochemical sensor was constructed.The linear range of the prepared sensor for detecting sodium nitrite was 0.2～2000μmol/L,and detection limit was 0.08μmol/L,with good reproducibility and stability.The sensor was used for the detection of nitrite in pickled meat samples,with a recovery range of 98.1% to 104.0%,indicating that the accuracy of this method was very good,and the sensor could be used for nitrite analysis in pickled meat samples.
[1] Lee H,Dellatore S M,Miller W M,et al.Mussel-inspired surface chemistry for multifunctional coatings[J].Science,2007,318(5849):426-430.
[2] 孙二强,王昭旭,刘佳莉,等.贻贝仿生化学聚多巴胺改性纳米吸附材料研究进展[J].化工新型材料,2022,50(12):61-67.
[3] Ji X,Yi B,Xu Y,et al.A novel fluorescent biosensor for adenosine triphosphate detection based on the polydopamine nanospheres integrating with enzymatic recycling amplification[J].Talanta,2017,169:8-12.
[4] Park J,Brust T F,Lee H J,et al.Polydopamine-based simple and versatile surface modification of polymeric nano drug carriers[J].ACS Nano,2014,8(4):3347-3356.
[5] Liu Y,Zhao Y,Fan Q,et al.Aptamer based electrochemiluminescent thrombin assay using carbon dots anchored onto silver-decorated polydopamine nanospheres[J].Microchimica Acta,2018,185(2):85.
[6] 黄应祥,汪杨,孔雀,等.聚吡咯/银/聚多巴胺抗菌导电蚕丝织物的制备及性能研究[J].化工新型材料,2023,51(4):264-274.
[7] 张林,尹华斌,骆健俊,等.基于电聚合多巴胺-透明质酸复合膜界面构建过氧化氢电化学传感器[J].分析化学,2013(4):534-539.
[8] 赵彦华,张晨韵,张胜男,等.聚多巴胺仿生纳米微球用于构建电化学免疫传感器[J].分析测试技术与仪器,2015(2):63-68.
[9] 黄诗思,曾延波,杨义文,等.基于聚多巴胺包覆的Zr-MOF的电化学传感器对双酚A的检测[J].分析测试学报,2021,40(10):1446-1452.
[10] Bahadoran Z,Ghasemi A,Mirmiran P,et al.Nitrate-nitrite-nitrosamines exposure and the risk of type 1 diabetes:a review of current data[J].World Journal of Diabetes,2016,7(18):433-440.
[11] 徐艺伟,朱丹,石吉勇,等.原位合成铜基金属有机框架差分脉冲伏安法快速检测肉制品中亚硝酸盐[J].食品科学,2021,42(16):267-272.
[12] 杨建飞,马倩,左勇,等.亚硝酸盐快速检测试剂研究及其在酱腌菜中的应用[J].食品科学,2021,42(18):321-328.
[13] 毛燕,包宇,韩冬雪,等.亚硝酸盐电化学传感器研究进展[J].分析化学,2018,46(2):147-156.
[14] 田曼丽,田亮亮.片状Co3O4多孔材料的制备及其在葡萄糖电化学传感器中的应用[J].化工新型材料,2020,48(9):264-268.
[15] 周瑶万起展王燕华,等.基于Nafion共聚还原氧化石墨烯/聚苯胺复合材料的H2O2电化学传感器[J].化工新型材料,2020,48(10):192-196.
[16] 羌越,陈璐,张静.碳量子点/金属有机骨架材料修饰玻碳电极用于双酚A的电化学检测[J].理化检验——化学分册,2021,57(1):7-14.
[17] Zhang M L,Huang J Z,Cui W W,et al.Kinetic studies of microfabricated biosensors using local adsorption strategy[J].Biosensors and Bioelectronics,2015,74(12):8-15.
[18] Huang S S,Liu L,Mei L P,et al.Electrochemical sensor for nitrite using a glassy carbon electrode modified with gold-copper nanochain networks[J].Microchimica Acta,2016,183(2):791-797.
[19] Kung C W,Chang T H,Chou L Y,et al.Porphyrin-based metal-organic framework thin films for electrochemical nitrite detection[J].Electrochemistry Communications,2015,58(9):51-56.
[20] Sun C X,Pan W X,Zheng D Y,et al.An electrochemical sensor for nitrite using a glassy carbon electrode modified with Cu/CBSA nanoflower networks[J].Analytical Methods,2019,11(39):4998-5006.
[21] Zhang Y,Nie J T,Wei H Y,et al.Electrochemical detection of nitrite ions using Ag/Cu/MWNT nanoclusters electrodeposited on a glassy carbon electrode[J].Sensors and Actuators B:Chemical,2018,258(4):1107-1116.
