针对耐药型细菌感染等疾病问题,利用共价固定法将光敏剂竹红菌素负载于细菌纤维素/壳聚糖复合材料上,制备具有光动力抗菌功能的纳米纤维膜。借助扫描电子显微镜、X射线衍射仪、傅里叶变换红外光谱仪和热重分析仪等手段分析比较固载竹红菌素前后纤维膜的形貌、结晶度、化学结构和热稳定性,并研究固载竹红菌素前后纤维膜对金黄色葡萄球菌和大肠杆菌的抗菌能力。结果表明:固载竹红菌素后,结晶度和热稳定性略有降低;在暗室条件下,纤维膜对金黄色葡萄球菌和大肠杆菌均具有良好的抗菌效果,在光照条件下,竹红菌素与壳聚糖的协同抗菌作用,使纤维膜对金黄色葡萄球菌的抗菌率提至99.9999%。
To meet the challenge of drug-resistant bacterial infection,the hypocrellin photosensitizer was covalently loaded on the bacterial cellulose/chitosan composite membrane to prepare the photodynamic antibacterial nanofiber membrane.The morphology,crystallinity,chemical structure and thermostability of the membrane before and after the addition of Hc were compared by scanning electron microscopy,X-ray diffraction,fourier transform infrared spectrometry and thermogravimetric analysis.The antibacterial ability to S.aureus and E.coli were also investigated to explore its photodynamic efficiency.The results shown that the addition of Hc slightly decreased their crystallinity and thermostability.The membrane shown good antibacterial effect against S.aureus and E.coli in the dark.Furthermore,the antibacterial effect against S.aureus reached 99.9999% under illumination due to the synergy of hypocrellin and chitosan.
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