新材料与新技术

荧光共轭聚合物纳米微球的制备及光动力抗菌研究

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  • 1.常州大学材料与工程学院,常州213164;
    2.常州大学制药与生命科学学院,常州213164
刘丽丽(1994-),女,硕士,主要研究方向为聚合物杀菌材料,E-mail:885122632@qq.com。

收稿日期: 2020-04-02

  修回日期: 2021-03-16

  网络出版日期: 2021-06-29

基金资助

江苏省六大人才高峰创新团队(SWYY-CXTD-001)

Study on preparation and photodynamic antibacterial property of fluorescent conjugated polymer nanosphere

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  • 1. Shool of Materials Science and Engineering,Changzhou University,Changzhou 213164;
    2. School of Pharmaceutical,Engineering and Life Science,Changzhou University,Changzhou 213164

Received date: 2020-04-02

  Revised date: 2021-03-16

  Online published: 2021-06-29

摘要

通过水包油的微乳液聚合法,以异丙基丙烯酰胺和甲基丙烯酰氧乙基三甲基氯化铵为共聚单体,并与荧光共轭聚合物(FCP)混合后合成荧光共轭聚合物纳米微球(FCP-NPs),使用红外光谱、扫描电镜、透射电镜激光粒度仪等手段对微球进行了表征。结果表明:聚合物纳米微球包载了FCP,其平均粒径大约为119±13nm。FCP-NPs溶液在532nm激光照射下能生成单线态氧,具有光动力杀菌潜力。用FCP-NPs溶液分别与大肠杆菌、金黄色葡萄球菌及多重耐药菌耐甲氧西林金黄色葡萄球菌共培养,FCP-NPs在浓度较高时(>5mg/mL),激光照射下对于两种常见菌株的抑菌率大于95%,对耐药菌的抑菌率约80%。在无光照或者无FCP情况下,纳米微球仍然有一定的抑菌性能,因此FCP-NPs具有光动力-铵阳离子协同杀菌的功能。

本文引用格式

刘丽丽, 盛扬, 孙一新, 张嵘, 李坚, 徐德锋 . 荧光共轭聚合物纳米微球的制备及光动力抗菌研究[J]. 化工新型材料, 2021 , 49(6) : 112 -116 . DOI: 10.19817/j.cnki.issn 1006-3536.2021.06.025

Abstract

The oil-in-water microemulsion polymerization was used to synthesize fluorescent polymer nano-microspheres (FCP-NPs) with N-isopropylacrylamide and 2-(methacryloyloxy)ethyl trimethylammonium chloride as monomers mixed with a self-synthesized conjugated polymer.FCP-NPs were characterized by FT-IR,SEM,TEM,and laser particle size analyzer.The results showed that conjugated polymer was immobilized successfully in the nanospheres with the average size of 119±13nm in diameter.The FCP-NP solution was found generating singlet oxygen after exposing to 532nm laser light showing photodynamic antibacterial ability.Thus E.coli,S.aureus and multi-antibiotic resistant bacteria MRSA were used for test.The results indicated that the inhibition rate of FCP-NPs to two common bacterial strains was more than 95%,and to MRSA was about 80% when the concentration of FCP-NPs was high (>5mg/mL).In the absence of light or FCP,the nano microspheres still showed antibacterial properties.Therefore,FCP-NPs had the function of photodynamic-ammonium cation synergistic sterilization.

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