优化设计的功能水凝胶是一种优良的药物缓释载体。以聚乙烯醇(PVA)和羧甲基壳聚糖(CMCS)为原料,以两种物理交联方法相结合,制备了具有互穿网络结构的PVA-CMCS复合水凝胶。通过形貌特征分析、X射线分析、热重分析、红外分析和溶胀性能等一系列表征考察了复合凝胶的物理化学性能。探索了PVA-CMCS复合水凝胶的最佳制备条件,并进行了载药和释药行为研究。结果发现:CMCS浓度、甘油磷酸钠(GP)用量及冻融次数均会影响复合水凝胶的形态和溶胀性能。其中5% PVA与3% CMCS等量混合、加入0.6g GP、冻融4次的复合水凝胶,在形态和溶胀上的结果最好。然后分别以大分子药物牛血清白蛋白(BSA)和小分子药物盐酸二甲双胍(MH)为模型药物,经释药研究表明,该复合水凝胶具有明显的pH敏感性,而且对大分子药物表现出更好的缓释效果。该复合凝胶制备方法简单温和,在作为大分子药物缓控释输送载体方面具有潜在的应用前景。

The optimized functional hydrogel is an excellent sustained-release drug carrier.Polyvinyl alcohol (PVA)/carboxymethyl chitosan (CMCS) composite hydrogels with interpenetrating network were prepared.A series of characterizations,such as X-ray analysis,thermogravimetric analysis,infrared analysis and swelling property,were carried out.The optimal performance conditions of the hydrogel were investigated,and the drug release experiment was carried out by preparing PVA-CMCS loaded hydrogel.The results showed that the morphology and swelling properties of the hydrogels were affected by the concentration of CMCS,the dosage of sodium glycerophosphate (GP) and the number of freeze-thaw times in PVA-CMCS hydrogels.If the optimal preparation condition was that the volume of 5% PVA was equal mixing to the volume of 3% CMCS and 0.6g GP,as well as freeze-thawed for 4 times,the hydrogels had the best results in morphology and swelling behavior.Under these conditions,the drug-loaded hydrogels containing macromolecular bovine serum albumin and small metformin hydrochloride were prepared respectively.The results of drug release experiments showed that PVA-CMCS exhibited notably pH sensitivity as well as good performance in sustained release of macromolecular drugs.Furthermore,PVA-CMCS composite hydrogels prepared by the simple and mild process had great potential to be used as sustained drug delivery carriers for macromolecular drugs.