丝素蛋白(SF)是从蚕丝中提取的一种具有嵌段结构的高相对分子质量蛋白,生物相容性良好,可用于稳定银纳米粒子(Ag NPs)。以硝酸银、硼氢化钠为原料,SF为稳定剂制备了丝素蛋白包覆的银纳米粒子(SF-Ag NPs),以柠檬酸钠、牛血清蛋白(BSA)和聚乙烯吡咯烷酮(PVP)为稳定剂分别制备了不同的Ag NPs样品作为对照,通过紫外-可见分光光度计研究了SF-Ag NPs在不同pH溶液及不同浓度盐离子体系中的稳定性。结果表明:SF-Ag NPs在pH=3～11以及不同浓度盐离子(NaCl 50～400mmol/L,CaCl2 12.5～100mmol/L)中稳定性良好。稳定机理研究表明,空间位阻效应以及SF中氮原子与Ag NPs的结合力使得SF具有良好的稳定作用。抗菌测试结果表明SF-Ag NPs具有良好的抗菌性。
Protein silk fibroin (SF),which belongs to a class of unique,biocompatible,block copolymer-like proteins,was investigated for the stabilization of Ag NPs in an aqueous dispersion.Three representative stabilizers,including sodium citrate,bovine serum albumin (BSA) and polyvinyl pyrrolidone(PVP),were utilized as control.The effects of pH value,salt type and concentration on the stability of SF capped Ag NPs (SF-Ag NPs) for against aggregation was confirmed over a wide pH value ranged from 3 to 11 and different salt ion (NaCl 50～400mmol/L,CaCl2 12.5～100mmol/L) concentration through UV-Vis spectroscopy.The stabilization effect of SF may be attributed to the steric effect and the nitrogen atoms of SF.Moreover,SF-Ag NPs maintained high antibacterial activity.Thus,SF-Ag NPs can be potentially used in biomedical applications.
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基金资助
国家自然科学基金(青年基金)(51303124)
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