将2,2,6,6-四甲基哌啶-1-氧化物自由基(TEMPO)氧化纳米纤维素(NFC)与庆大霉素(GM)进行物理共混,采用冷冻干燥法制备出孔结构可调控的载药NFC气凝胶。探讨了NFC均质次数和羧基含量对GM/NFC气凝胶的孔结构、溶胀性、药物释缓性能以及抗菌效果的影响。结果表明:所制备的GM/NFC气凝胶的孔径范围为150～450μm,孔隙率均在96%以上。体外释放实验结果表明:药物累积释放率随气凝胶平均孔径的增加而升高,在420min内可控制在74.15%～90.19%之间。药物体外释药行为符合Peppas方程,药物释放以扩散控释型为主。同时GM/NFC气凝胶显示出较好的抗菌特性,对标准金黄色葡萄球菌的抑菌圈宽度范围为13.31～16.98mm。
Gentamicin(GM)/NFC sustained-release aerogels with tunable pore structure were prepared by freeze-drying the mixture of TEMPO oxidized NFC with GM.The influences of carboxyl content of NFC and homogenization times on the pore structure,swelling property,drug release property and antibacterial effect of GM/NFC aerogels were investigated.The obtained GM/NFC aerogels had porous network structures,with porosity above 96% and pore sizes in the range of 150～450μm.The drug release rate of GM/NFC aerogels were improved with increasing the average pore size,and controlled between 74.15% and 90.19% in 420min.The results of in vitro release tests revealed that drug release behaviors from GM/NFC aerogels fitted with Peppas model,indicating that drug release were mainly controlled by diffusion.Simultaneously,GM/NFC aerogels showed excellent antibacterial properties,with the inhibition zone′s width of standard staphylococcusaureus in the range of 13.31～16.98mm.
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基金资助
国家自然科学基金(51875214);华南理工大学制浆造纸工程国家重点实验室基金(2016PY01和2015C09);广州市科技计划项目(201804010452)
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