糖尿病是一种严重威胁人体健康且无法治愈的疾病。然而,目前缺乏稳定性好、成本低的血糖检测方法,设计并制备了一种新型的基于石英晶体微天平(QCM)的血糖检测技术,选择氨基苯硼酸作为葡萄糖的识别单元,将氨基苯硼酸与氧化多壁碳纳米管(MWCNTs)结合,然后再涂覆至金电极上。利用QCM检测薄膜与葡萄糖的结合特性。结果表明:该薄膜能够检测生理条件下(pH=7.0～7.5)的葡萄糖浓度变化,整个检测范围为1.1～33.3mmol/L,且具有良好的可再生性,并对其他干扰糖(果糖、麦芽糖、核糖、乳糖、木糖、蔗糖、半乳糖、甘露糖)有较好的选择性。这项工作为检测血糖提供了一种新方法。
Diabetes is a serious threat to human health,and cannot be cured.However,there is a lack of stable and low cost blood glucose detection system.A new QCM blood glucose detection system was designed and prepared.Aminophenylboronic acid was chose as recognition unit for detect glucose.Then aminophenylboronic acid was combined with the oxidized multiwall carbon tube and finally coated on the quartz crystal plate.Then QCM was used to detect the binding properties of membrane to glucose.The results showed that the film could detect the change of glucose concentration under physiological conditions (pH=7.0～7.5).The whole detection range was from 1.1～33.3mmol/L.It also had good reproducibility and excellent selectivity to other interfering sugars (fructose,maltose,ribose,lactose,xylose,sucrose,galactose and mannose).This work provided a new method for continuous measurement of blood glucose.
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基金资助
国家自然科学基金(51503005);北京市科技北京百名领军人才工程(Z16111000490000);北京市教育委员会科技计划一般项目(SQKM201710012004)
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