Polyelectrolyte brushes have attracted much attention because of their good compatibility with biological macromolecules.Used styrene as raw material and polystyrene nano-magnetic core particles (MPS) coated with Fe3O4 were prepared by microemulsion polymerization.Acrylic acid (BA) was used as photoinitiator,acrylic acid (AA) was polyelectrolyte brush and magnetic nanosphere brush (MSPB) with a long chain length controllable was prepared.The effects of BA,AA and polymerization time on the brush chain length of polyelectrolyte were investigated,and the preparation conditions of MSPB were optimized.The structure and properties of MSPB were characterized by DLS,TG and SEM.The results showed that the optimum conditions were as follows:ST 2.5g,BA/ST=0.1(molar ratio),AA/ST=0.9,polymerizaion time 2.0h,the magnetic particle size of MSPB was 152nm,PAA chain length was 160 nm,and with superparamagnetism.The immobilized enzyme activity was 1.75×104u/g carrier and the immobilized enzyme content was 42.9mg/g,the recovery rate of enzyme activity was 93%.It showed that MSPB can provide a good carrier for immobilized enzymes.
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基金资助
国家自然科学基金(51372043);广西石化资源加工及过程强化技术重点实验室项目(2016K010和2015Z003);广西大学大学生实验创新基金(201610593156)
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