The preparation of three types of fluorescent nanoparticles were studied by applying Stöber method,inverse microemulsion polymerization and in-situ polymerization,respectively:(1)carboxyl functional SiO2 fluorescent nanoparticles,(2)polyacrylamide-co-polyacrylic acid polymer (PAAAM) fluorescent nanoparticles,(3)core shell structure SNP@PAAAM fluorescent nanoparticles.All these nanoparticles were well dispersed in water.Transmission electron microscope,infrared spectroscopy,fluorescence spectrophotometry,dynamic light scattering analysis and zeta potentials were used to characterize the nanoparticles.Fluorescence spectroscopy showed that three NPs with RhX 1wt% all had highest fluorescent intensity.SNP@PAAAM nanoparticles and rodamine red X(RhX) were conjugated to obtain SNP@PAAAM@RhX,which can be well separated from the water and also had good water dispersion.The zeta potential was -22.7mV,and the average particle size was 90nm.Three prepared fluorescence nanoparticles can be widely applied in important rapid biomarker diagnosis and disease tracking.
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基金资助
国家自然科学基金(21401051,21501054);湖北省自然科学基金(2014CFA080);湖北省科技厅高等学校优秀中青年科技创新团队计划(T201705);湖北省“楚天学者”计划(2013);湖北省百人计划(2013);湖北省大学生创新创业训练计划(201410500021)
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