以聚天冬氨酸(PASP)与壳聚糖季铵盐(HTCC)为基元,运用层层组装技术制备了可以释放双药物的层层组装聚合物膜。通过先采用浸泡的形式负载盐酸柔红霉素(DNR)并喷涂阻隔层,再将盐酸万古霉素(VAN)以复合物PASP-VAN的形式与HTCC层层组装的方法,实现了DNR与VAN在膜中的共同负载。探讨了阻隔层的周期数对VAN与DNR释放效果的影响,结果表明,阻隔层可以延缓VAN与DNR的释放速率,VAN与DNR在生理盐水中的释放时间分别持续1680min和2700min,累积释放量分别为2.26mg和0.20mg。
The multilayer films were fabricated by LbL assembly of polyaspartic acid (PASP) and chitosan quaternary ammonium (HTCC).Daunorubicin hydrochloride (DNR) molecules were firstly loaded into film by post-diffusion process.Then the capping layers were assembled on the film by using spray-coating.The second kind of molecules vancomycin hydrochloride (VAN) was loaded into films by first complexing with PASP to prepare PASP-VAN polyeletrolyte complexes,and then PASP-VAN was deposited with the oppositely charged polyelectrolyte to prepare films.At last,two kinds of drugs VAN and DNR were loaded into one multilayer films.The effect of the number of capping layers on the release time of DNR and VAN molecules were studied,the results showed that the capping layers could effectively slow down the release rate of DNR and VAN.The release experiment of dual drug delivery system indicated that DNR and VAN were released in physiological saline,VAN molecules released 1680 min and DNR molecules released last for 2700min.The cumulative release amount of VAN was 2.26mg,the accumulate release amount of DNR was 0.20 mg.
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基金资助
国家自然科学基金(21304066);太原理工大学2012年校青年基金(2012L058)
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