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摘要
采用Hummers法,以石墨为原料制备氧化石墨烯(GO)。再以辣根过氧化物酶(HRP)和过氧化氢(H2O2)的酶催化反应,诱导制备了多孔石墨烯(PGR)。采用傅里叶红外光谱、扫描电子显微镜和电化学分析法对其结构、形貌和性能表征。结果表明:10mg/mL GO溶液加入80μL 5mg/mL HRP后,再每日加入20μL(1×10-3)mol/L H2O2反应10d可以获得最佳的多孔结构。基于此构置的生物催化诱导型葡萄糖传感器,反应25min时,可在电位为0.98V处呈现Au氧化峰,使其可用于葡萄糖的高灵敏检测。
Abstract
Graphene oxide (GO) was prepared by modified Hummers method with graphite as raw material.Then,porous graphene (PGR) was induced by the reaction between horseradish peroxidase (HRP) and hydrogen peroxide (H2O2).Fourier transform infrared spectroscopy,scanning electron microscopy and electroanalytical method were employed to character the product.The results indicated that PGR prepared at the condition which added 80μL of 5mg/mL HRP into 10mg/mL GO,followed by 20μL of (1×10-3) mol/L H2O2 each day for 10 days.The biological catalysis induced type of glucose biosensor based on PGR,when reaction time 25min,the potential at 0.98V showed an oxidative peak of Au.PGR can be used for the determination of glucose with high sensitivity.
关键词
生物传感
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酶催化
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多孔石墨烯
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辣根过氧化物酶
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葡萄糖氧化酶
Key words
biosensing
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enzyme catalysis
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porous graphene
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horseradish peroxidaseglucose oxidase
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glucose oxidase
酶催化诱导制备多孔石墨烯的研究[J].
化工新型材料, 2017, 45(9): 77-79 DOI:
[1] 马文石,周俊文,程顺喜.石墨烯的制备与表征[J].高校化学工程学报,2010,24(4):719-722.
[2] 赵闯.化学还原氧化石墨烯及其复合物的制备和应用研究[D].郑州:河南大学,2013.
[3] 冯晓苗,李瑞梅,杨晓燕,等.新型碳纳米材料在电化学中的应用[J].化学进展,2012,24(11):2158-2166.
[4] Kotchey G P,Allen B L,Vedala H,et al.The enzymatic oxidation of graphene oxide[J].ACS Nano,2011,5(3):2098-2108.
[5] Chen Z P,Peng Z F,Luo Y,et al.Successively amplified electrochemical immunoassay based on biocatalytic deposition of silver nanoparticles and silver enhancement[J].Biosensors and Bioelectronics,2007,23(4):485-491.
[6] Wang S,Xue J,Ge X,et al.Biomimetic synthesis of silica nanostructures with controllable morphologies and sizes through tuning interfacial interactions[J].Chemical Communications,2012,48(75):9415-9417.
[7] 何亚萍.基于形貌可控纳米材料的电化学传感研究[D].西安:西北大学,2013.
[8] Hummers S,Offeman R.Preparation of graphitic oxide[J].J Am Chem Soc,1958,80(6):1339-1339.
基金资助
国家自然科学基金(21445004、21545014、21643014和21275116);西安市科技计划项目(CXY1443WL27);陕西省教育厅重点实验室访问学者项目(14JS095);西安文理学院博士启动基金(06005017);陕西省分析化学重点学科(09009001);大学生创新创业训练计划项目(201611080008,201613)
