The intervention of small organic molecules (EN) will be used as a new buffer system.At a suitable concentration of EN,the protonation degree of EN was changed by changing the pH value in the buffer solution and hybridization system,the ionization intensity of the solution was further changed,and a series of pH-related programmable assembly products were developed.Through the double helix structure of DNA chain,the programmed assembly of gold nanoparticles mediated by DNA was realized.H+(HCl) was added to accurately adjust the pH value of the buffer solution and the hybridization system system,thereby adjusting the density of outer layer “satellite” nanoparticles in the core-shell satellite nano-assembly structure.The product was purified by combining agarose gel electrophoresis characterization and separation technology,and characterized by a transmission electron microscope to realize controllable programmed assembly of gold nanoparticles.
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