以L-色氨酸为印迹分子,S-2-巯基丙酸为手性功能单体,分别制备了25个L-色氨酸分子印迹复合膜(MICM1～25)及相应的非印迹复合膜(NICM1～25)。采用扫描电镜(SEM)表征最优印迹复合膜(MICM5)及相应的非印迹复合膜(NICM5)的外观形态,通过等温吸附模型对印迹复合膜的吸附性能进行评价,并结合高效液相色谱法(HPLC法)分析MICM5对外消旋体DL-色氨酸的手性拆分能力。结果表明:以聚四氟乙烯膜为支撑膜,印迹分子、功能单体和交联剂的摩尔比为1∶8∶90,甲醇∶水(1∶1,体积比)为致孔剂可制备出较优的分子印迹复合膜,其对外消旋体DL-色氨酸有较好的手性拆分能力,拆分因子达到2.41。相对于常用功能单体制备的L-色氨酸分子印迹复合膜,采用S-2-巯基丙酸为手性功能单体制备的L-色氨酸分子印迹复合膜具有更高的亲和性和更好的手性拆分能力。
Using L-tryptophan as imprinted molecule and S-2-mercaptopropionic acid as chiral functional monomer,25 L-tryptophan molecular imprinted composite membranes (MICM1～25) and corresponding non-imprinted composite membranes (NICM1～25) were prepared,respectively.The appearance of the optimal composite membrane (MICM5) and the corresponding non-imprinted composite membrane (NICM5) were characterized by scanning electron microscope (SEM),and the adsorption performance of the membrane was evaluated by the isothermal adsorption model.The chiral resolution ability of the racemic DL-tryptophan was also investigated by high performance liquid chromatography.The results showed that the optimal experimental conditions were as follows:the polytetrafluoroethylene membrane was used as the base membrane,the molar ratio of imprinting molecule,functional monomer and crosslinking agent was 1∶8∶90,the porogen was methanol∶water (v∶v,1∶1),optimal molecularly imprinted composite membrane was prepared.It showed a desired chiral resolvability for the racemic DL-tryptophan with a resolution factor of 2.41.The L-tryptophan molecular imprinted composite membrane prepared with S-2-mercaptopropionic acid as functional monomer had higher affinity and better chiral resolution ability than the prepared by common functional monomers.
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