Abstract: Protein silk fibroin (SF),which belongs to a class of unique,biocompatible,block copolymer-like proteins,was investigated for the stabilization of Ag NPs in an aqueous dispersion.Three representative stabilizers,including sodium citrate,bovine serum albumin (BSA) and polyvinyl pyrrolidone(PVP),were utilized as control.The effects of pH value,salt type and concentration on the stability of SF capped Ag NPs (SF-Ag NPs) for against aggregation was confirmed over a wide pH value ranged from 3 to 11 and different salt ion (NaCl 50~400mmol/L,CaCl2 12.5~100mmol/L) concentration through UV-Vis spectroscopy.The stabilization effect of SF may be attributed to the steric effect and the nitrogen atoms of SF.Moreover,SF-Ag NPs maintained high antibacterial activity.Thus,SF-Ag NPs can be potentially used in biomedical applications.
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